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A1USA2 (ISPDF_BARBK) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional enzyme IspD/IspF

Including the following 2 domains:

  1. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
    EC=2.7.7.60
    Alternative name(s):
    4-diphosphocytidyl-2C-methyl-D-erythritol synthase
    MEP cytidylyltransferase
    Short name=MCT
  2. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
    Short name=MECDP-synthase
    Short name=MECPS
    EC=4.6.1.12
Gene names
Name:ispDF
Ordered Locus Names:BARBAKC583_0540
OrganismBartonella bacilliformis (strain ATCC 35685 / KC583) [Complete proteome] [HAMAP]
Taxonomic identifier360095 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (IspF) By similarity. HAMAP MF_01520

Catalytic activity

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520

2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520

Cofactor

Divalent metal cations By similarity. HAMAP MF_01520

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.

Sequence similarities

In the N-terminal section; belongs to the IspD family.

In the C-terminal section; belongs to the IspF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 397397Bifunctional enzyme IspD/IspF HAMAP MF_01520
PRO_0000292849

Regions

Region1 – 2362362-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
Region237 – 3971612-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520

Sites

Metal binding2431Divalent metal cation By similarity
Metal binding2451Divalent metal cation By similarity
Metal binding2771Divalent metal cation By similarity
Site151Transition state stabilizer By similarity
Site241Transition state stabilizer By similarity
Site1551Positions MEP for the nucleophilic attack By similarity
Site2121Positions MEP for the nucleophilic attack By similarity
Site2691Transition state stabilizer By similarity
Site3681Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
A1USA2 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 62EE301AA230811C

FASTA39743,941
        10         20         30         40         50         60 
MSIAAIILAA GRGSRAGSLQ NGPKQYRFLG REPVICHTIR CFLQHPAITN IILVIHPDDY 

        70         80         90        100        110        120 
QICANAIADL KDHLTIIEGG ETRQISTLQG LRALKNVKPN YVHIHDGARP FIGNELLEQI 

       130        140        150        160        170        180 
HTSINHKEGI LPVLSVSDTL KYVSNTHHVL ETIPRTNLYR AQTPQCFPFE LILAAHEKAM 

       190        200        210        220        230        240 
QSHKQDFTDD SAIAEWFGIP IRTIPGDPNN IKITWPADLE TARSYLLKGM QIFPDIRTGN 

       250        260        270        280        290        300 
GYDVHSFEDG HYITLCGIKI PFEKKLNGHS DADVALHALT DALLATQGAG DIGTHFPPSD 

       310        320        330        340        350        360 
PQWKNASSEI FLHHAIEIIK KAEGRIANVD ITLIAEKPKI GPYRHIMTEN LMKILKISTD 

       370        380        390 
RISIKATTNE KLGFIGREEG IAALATATVI YPGEIPK

« Hide

References

[1]Hendrix L., Mohamoud Y., Radune D., Shvartsbeyn A., Daugherty S., Dodson R., Durkin A.S., Harkins D., Huot H., Kothari S.P., Madupu R., Li J., Nelson W.C., Shrivastava S., Giglio M.G., Haft D., Selengut J., Fraser-Ligget C., Seshadri R.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35685 / KC583.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000524 Genomic DNA. Translation: ABM45054.1.
RefSeqYP_988855.1. NC_008783.1.

3D structure databases

ProteinModelPortalA1USA2.
ModBaseSearch...

Protein-protein interaction databases

STRINGA1USA2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4684478.
GenomeReviewsGene locus BARBAKC583_0540 in contig CP000524_GR.
KEGGbbk:BARBAKC583_0540.
NMPDRfig|360095.3.peg.229.
PATRIC20537353. VBIBarBac6912_0524.
TIGRBARBAKC583_0540.

Phylogenomic databases

eggNOGCOG1211.
HOGENOMHBG672839.
OMAIVLIHDA.
ProtClustDBCLSK927085.

Enzyme and pathway databases

BioCycBBAC360095:BARBAKC583_0540-MONOMER.

Family and domain databases

HAMAPMF_01520. IspDF.
[Tree]
InterProIPR023423. IpsF_dom.
IPR001228. ISPD_synthase.
IPR003526. MECDP_synthase.
IPR020555. MECDP_synthase_CS.
[Graphical view]
Gene3DG3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.
KOK12506.
PfamPF01128. IspD. 1 hit.
PF02542. YgbB. 1 hit.
[Graphical view]
SUPFAMSSF69765. YgbB_synth. 1 hit.
TIGRFAMsTIGR00453. IspD. 1 hit.
TIGR00151. IspF. 1 hit.
PROSITEPS01295. ISPD. False negative.
PS01350. ISPF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameISPDF_BARBK
AccessionPrimary (citable) accession number: A1USA2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: February 6, 2007
Last modified: January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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