A1US27 (DAPA_BARBK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	BARBAKC583_0464

Organism

Bartonella bacilliformis (strain ATCC 35685 / KC583) [Complete proteome] [HAMAP]

Taxonomic identifier

360095 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000050165
Regions
	Region	48 – 49	2	Pyruvate binding By similarity
Sites
	Active site	162	1	Schiff-base intermediate with substrate By similarity
	Binding site	106	1	Pyruvate By similarity
	Site	133	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1US27 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 40D1F33182098007	FASTA	294	31,618
	10 20 30 40 50 60 MLKGALTALI TPFNENGSVD EETFCNFIEW QITQGIDGLI PAGTTGESPT LSHEEHKKVI 70 80 90 100 110 120 ELCVEKVAKR VPVVAGAGSN STDEAVELAQ HAKKAGADAV LVVTPYYNKP NQEGIYSHFA 130 140 150 160 170 180 TIAKAVSIPI VIYNIPGRSV VDMAVETMKD LCQNFKNIIG VKDSTGNIER VSEQREKCGK 190 200 210 220 230 240 DFVQLSGDDQ TALGFNAHGG VGCVSVASNV APKLCAELYA ACRSGDYKTA LELNDRLMPL 250 260 270 280 290 NRSIFIEPSP SGIKYAVAKL GFCRDTVRLP LVPLKDTTKE IIDAALQHAG LIKE

References

[1]

Hendrix L., Mohamoud Y., Radune D., Shvartsbeyn A., Daugherty S., Dodson R., Durkin A.S., Harkins D., Huot H., Kothari S.P., Madupu R., Li J., Nelson W.C., Shrivastava S., Giglio M.G., Haft D., Selengut J., Fraser-Ligget C., Seshadri R. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35685 / KC583.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000524 Genomic DNA. Translation: ABM44638.1.
RefSeq	YP_988780.1. NC_008783.1.
3D structure databases
ProteinModelPortal	A1US27.
SMR	A1US27. Positions 1-292.
ModBase	Search...
Protein-protein interaction databases
STRING	A1US27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4684902.
GenomeReviews	Gene locus BARBAKC583_0464 in contig CP000524_GR.
KEGG	bbk:BARBAKC583_0464.
NMPDR	fig|360095.3.peg.158.
PATRIC	20537197. VBIBarBac6912_0446.
TIGR	BARBAKC583_0464.
Phylogenomic databases
eggNOG	COG0329.
HOGENOM	HBG358848.
OMA	HGCISVV.
ProtClustDB	PRK03170.
Enzyme and pathway databases
BioCyc	BBAC360095:BARBAKC583_0464-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_BARBK

Accession

Primary (citable) accession number: A1US27

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program