Reviewed, UniProtKB/Swiss-Prot A1URX2 (ISPH_BARBK)

Names and origin

Protein names

Recommended name:
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
    EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	BARBAKC583_0406

Organism

Bartonella bacilliformis (strain ATCC 35685 / KC583) [Complete proteome] [HAMAP]

Taxonomic identifier

360095 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity.
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity.
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the ispH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021090

Sequences

	Sequence		Length	Mass (Da)
	A1URX2-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: CEAC5D510642A61C	FASTA	349	38,493
	10 20 30 40 50 60 MSLLPPLTIR LCSPRGFCAG VDRAIQIVIL ALKKYGAPVY VRHEIVHNRY VVEGLQQRGA 70 80 90 100 110 120 VFVEELDEIP EKHRGQPVVF SAHGVPKSVS EKASLYNLFY LDATCPLVSK VHKQAIRHQR 130 140 150 160 170 180 HGRHVILIGH SGHPEVIGTM GQLEKGAVTL IETVEDALYY QPENPNKLGF VTQTTLSVED 190 200 210 220 230 240 TAGILDVLQK RFPALESPAT ESICYATTNR QEAVKAAALG SDLFLIVGSS NSSNARRLVE 250 260 270 280 290 300 VAERFGARQS ILVQRADDID FDRLGTLSVI SLSAGASAPE IIVDEIITAF RKRYNVKIEL 310 320 330 340 AETAVETQQF LVNRELRDVI LTPQDMAFVS GGVKTGRNDD TQMLKIEEK

References

[1]

Hendrix L., Mohamoud Y., Radune D., Shvartsbeyn A., Daugherty S., Dodson R., Durkin A.S., Harkins D., Huot H., Kothari S.P., Madupu R., Li J., Nelson W.C., Shrivastava S., Giglio M.G., Haft D., Selengut J., Fraser-Ligget C., Seshadri R. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000524 Genomic DNA. Translation: ABM44637.1.
RefSeq	YP_988725.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1URX2.
Genome annotation databases
GeneID	4684938.
GenomeReviews	Gene locus BARBAKC583_0406 in contig CP000524_GR.
KEGG	bbk:BARBAKC583_0406.
NMPDR	fig|360095.3.peg.454.
TIGR	BARBAKC583_0406.
Phylogenomic databases
OMA	PVIFSAH.
Family and domain databases
HAMAP	MF_00191. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. ispH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_BARBK

Accession

Primary (citable) accession number: A1URX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)