Reviewed, UniProtKB/Swiss-Prot A1URV3 (ISPE_BARBK)

Names and origin

Protein names

Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	BARBAKC583_0387

Organism

Bartonella bacilliformis (strain ATCC 35685 / KC583) [Complete proteome] [HAMAP]

Taxonomic identifier

360095 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000007813
Regions
	Nucleotide binding	106 – 116	11	ATP Potential
Sites
	Active site	20	1	By similarity
	Active site	148	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1URV3-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 90F0E209352AB599	FASTA	304	33,157
	10 20 30 40 50 60 MISTVQDSLE GHFHVLTPIK LNLALHIVGQ RTDGYHLLES LVYFSLSGDC LSYAPCERDR 70 80 90 100 110 120 FILTGPFAKG LSCNSINLAV RARDFMHKTF PRSAKPSSFQ LMKTLPVASG IGGGSGDAAS 130 140 150 160 170 180 ILNILRQKWK LDCSCEELAE MSLALGADVP MCLFALEYQQ PLFVKGIGND VTRLEEACSI 190 200 210 220 230 240 AMVLVNHGQK IATETIFNAL EKRNHPPLKI NPVALKTVSS LVEVLQETRN DLFIPALKIA 250 260 270 280 290 300 PQLAEVLSTL DESGSLFSRM SGSGATCFGI FKDQQAAQKA AAFIKSMHPN WFVKSIITLG RSSQ

References

[1]

Hendrix L., Mohamoud Y., Radune D., Shvartsbeyn A., Daugherty S., Dodson R., Durkin A.S., Harkins D., Huot H., Kothari S.P., Madupu R., Li J., Nelson W.C., Shrivastava S., Giglio M.G., Haft D., Selengut J., Fraser-Ligget C., Seshadri R. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000524 Genomic DNA. Translation: ABM45227.1.
RefSeq	YP_988706.1.
3D structure databases
SMR	A1URV3. Positions 14-294.
ModBase	Search...
Protein-protein interaction databases
STRING	A1URV3.
Genome annotation databases
GeneID	4684924.
GenomeReviews	Gene locus BARBAKC583_0387 in contig CP000524_GR.
KEGG	bbk:BARBAKC583_0387.
NMPDR	fig|360095.3.peg.435.
TIGR	BARBAKC583_0387.
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	ELAMIMQ.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. [Graphical view]
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_BARBK

Accession

Primary (citable) accession number: A1URV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)