Reviewed, UniProtKB/Swiss-Prot A1URL3 (PYRD_BARBK)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	BARBAKC583_0293

Organism

Bartonella bacilliformis (strain ATCC 35685 / KC583) [Complete proteome] [HAMAP]

Taxonomic identifier

360095 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_1000100248
Sites
	Active site	171	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1URL3-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 27927ED80C6FE9DF	FASTA	362	40,239
	10 20 30 40 50 60 MSFFQCIGRS ALFMLDPEHA HNLAILWLKS GLNSHKRIVD KRLSVRVAGL KFENFMGLAA 70 80 90 100 110 120 GFDKNAEVVD AVFRLGFGFT EVGTVTPKPQ AGNAKPRLFR LKEDEAIINR MGFNNDGHQI 130 140 150 160 170 180 VHDRLQGRKK SDIVGVNIGA NKDTLNRIGD YVAGITCFYD VADYFTVNIS SPNTPGLRDL 190 200 210 220 230 240 QARDNLRLLI KEISQARSEN KKKHGFSIPI FLKLAPDLTE QELDDVAAEL SLSDFDGLVV 250 260 270 280 290 300 SNTTLFRQGL KKSAFMNEGG GLSGRPLFER STIVLAKMRQ KLGKDIAIIG VGGVWDAQTA 310 320 330 340 350 360 LEKIKAGADL VQFYSGMVYE GPDLAKNITK DVLQMMQQDG VDTIKAYRDH HVEKWAKFEL HL

References

[1]

Hendrix L., Mohamoud Y., Radune D., Shvartsbeyn A., Daugherty S., Dodson R., Durkin A.S., Harkins D., Huot H., Kothari S.P., Madupu R., Li J., Nelson W.C., Shrivastava S., Giglio M.G., Haft D., Selengut J., Fraser-Ligget C., Seshadri R. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000524 Genomic DNA. Translation: ABM44480.1.
RefSeq	YP_988616.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1URL3.
Genome annotation databases
GeneID	4683876.
GenomeReviews	Gene locus BARBAKC583_0293 in contig CP000524_GR.
KEGG	bbk:BARBAKC583_0293.
NMPDR	fig|360095.3.peg.350.
TIGR	BARBAKC583_0293.
Phylogenomic databases
OMA	AALNRMG.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_BARBK

Accession

Primary (citable) accession number: A1URL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)