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A1UR39 (SYL_BARBK) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BARBAKC583_0104
OrganismBartonella bacilliformis (strain ATCC 35685 / KC583) [Complete proteome] [HAMAP]
Taxonomic identifier360095 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBartonellaceaeBartonella

Protein attributes

Sequence length875 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

regulation of translational fidelity

Inferred from electronic annotation. Source: GOC

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 875875Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009295

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif633 – 6375"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6361ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1UR39 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: DF9B351DA23E27B8

FASTA87598,727
        10         20         30         40         50         60 
MTIERYNPRA QEQKWQAIWD EKKIFQTSHE DEGEKYYVLE MFPYPSGRIH MGHVRNYTMG 

        70         80         90        100        110        120 
DVVARYKRAK GMNVLHPMGW DAFGMPAENA AMQNKVHPKA WTYQNIAAMR KQLQKLGLSI 

       130        140        150        160        170        180 
DWSREFATCD VDYYHRQQMI FLDLYQKGLV VRKVAKVNWD PVDQTVLANE QVIDGRGWRS 

       190        200        210        220        230        240 
GALVEQRELT QWFFKITEFG EGLLARLDDL TEWPDKVRVM QKNWIGKSQG LYIRWALDKT 

       250        260        270        280        290        300 
QLPHNDGCEG FDEITCYSTR PDTLFGASFL ALSVDHPVAQ ALARNDEELR AFIEMCRCGS 

       310        320        330        340        350        360 
TTTEALETAE KQGFRTGVLA VHPLNPAVRL PVYIANFVLM DYGTGAIFGC PAHDQRDLDF 

       370        380        390        400        410        420 
ARKYDLPVQI VVAPKEAEEQ DFTLSDTAYT GDGVMINSDF LNGLTPKDAF EVVAQHLEKQ 

       430        440        450        460        470        480 
VLNGQPQGQK TVQFRLRDWG VSRQRYWGCP IPMIHCAACG VVPVPRADLP VVLPEDVTFD 

       490        500        510        520        530        540 
RPGNPLARHE TWQTVACPSC GQPAKRETDT MDTFVDSSWY YARFTAPWAQ EPTDQDIAAQ 

       550        560        570        580        590        600 
WLPVQQYIGG IEHAILHLLY ARFFMRAMKL AGHVNADEPF TGLFTQGMVV HETYRDAQGW 

       610        620        630        640        650        660 
VAPDEVSIVE QDGKRRAYKL TDQSEVTIGS IEKMSKSKKN VVDPDDIISS YGADTARWFM 

       670        680        690        700        710        720 
LSDSPPERDV IWSESGIEGA HRFVQRVWRC VALSAPILST IEPCAGHQGE ALELSKAAHR 

       730        740        750        760        770        780 
TLCAVEDDLE KLAFNRAVAR LYEFLNIMAP LLNTVADLDD EMKSALRQAM DFFCAMIAPM 

       790        800        810        820        830        840 
MPHLAEECHA ALGGKTLMSE CAWPVYDKAL IVEDSVTLPV QINGKKRGDV TVPVTADQAE 

       850        860        870 
IEQAVLALSF VQAQLAGKSV KKMIIVPKRI VNVVL 

« Hide

References

[1]Hendrix L., Mohamoud Y., Radune D., Shvartsbeyn A., Daugherty S., Dodson R., Durkin A.S., Harkins D., Huot H., Kothari S.P., Madupu R., Li J., Nelson W.C., Shrivastava S., Giglio M.G., Haft D., Selengut J., Fraser-Ligget C., Seshadri R.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 35685 / KC583.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000524 Genomic DNA. Translation: ABM45658.1.
RefSeqYP_988442.1. NC_008783.1.

3D structure databases

ProteinModelPortalA1UR39.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING360095.BARBAKC583_0104.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM45658; ABM45658; BARBAKC583_0104.
GeneID4684393.
KEGGbbk:BARBAKC583_0104.
PATRIC20536485. VBIBarBac6912_0104.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycBBAC360095:GHRY-104-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BARBK
AccessionPrimary (citable) accession number: A1UR39
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: February 19, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries