Reviewed, UniProtKB/Swiss-Prot A1UR21 (TRMD_BARBK)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BARBAKC583_0086

Organism

Bartonella bacilliformis (strain ATCC 35685 / KC583) [Complete proteome] [HAMAP]

Taxonomic identifier

360095 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000006452
Regions
	Region	131 – 136	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	111	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1UR21-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 2FB147EDB04D843F	FASTA	232	25,586
	10 20 30 40 50 60 MTFQAHVVTL YPEMFPGVLG HSLAGRALER GIWSLNTVQI RDFALDKHHS VDDTPAGGGA 70 80 90 100 110 120 GMVMRADVLV AAIDHCPPDL PRILLSPRGR PFNQAHARSL ASDRGVVLVC GRFEGVDERV 130 140 150 160 170 180 LQARKLEEIS IGDYILSGGE TAALVLLDAL VRLLPGVMGN QASGECESFE NGLLEHPHYT 190 200 210 220 230 RPPVFEGLEI PLVLTSGHHK AIADWRQEQA EMLTQKRRPD LYAIYNKNRR KT

References

[1]

Hendrix L., Mohamoud Y., Radune D., Shvartsbeyn A., Daugherty S., Dodson R., Durkin A.S., Harkins D., Huot H., Kothari S.P., Madupu R., Li J., Nelson W.C., Shrivastava S., Giglio M.G., Haft D., Selengut J., Fraser-Ligget C., Seshadri R. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000524 Genomic DNA. Translation: ABM44575.1.
RefSeq	YP_988424.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1UR21.
Genome annotation databases
GeneID	4684386.
GenomeReviews	Gene locus BARBAKC583_0086 in contig CP000524_GR.
KEGG	bbk:BARBAKC583_0086.
NMPDR	fig|360095.3.peg.1057.
TIGR	BARBAKC583_0086.
Phylogenomic databases
OMA	HRSVDDT.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BARBK

Accession

Primary (citable) accession number: A1UR21

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)