Reviewed, UniProtKB/Swiss-Prot A1UR09 (PUR9_BARBK)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	purH
Ordered Locus Names:	BARBAKC583_0074

Organism

Bartonella bacilliformis (strain ATCC 35685 / KC583) [Complete proteome] [HAMAP]

Taxonomic identifier

360095 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bartonellaceae › Bartonella

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP MF_00139
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 538	538	Bifunctional purine biosynthesis protein purH HAMAP MF_00139		PRO_1000018844

Sequences

	Sequence		Length	Mass (Da)
	A1UR09-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: C481387E41485124	FASTA	538	58,021
	10 20 30 40 50 60 MVVVSQNYPT PDLHRVRRAL LSVFDKTGLV EFAQELHAYG IELISTGGTS ESLIAAGLPV 70 80 90 100 110 120 KDVSEVTGFP QIMDGRVKTL HPLIHGGLLG VRDDPSHKAA MEKHGICGID LVVVNLYPFE 130 140 150 160 170 180 KTWQSGADSQ TIIENIDIGG PAMIRAAAKN HAYTGVVTAV SDYDVVLAEL KQHNGCLSFS 190 200 210 220 230 240 LRRQLAARAY EHTAAYDAAI AAWFAQDLEV EMPSWQNFSG HFESMMRYGE NPHQRAAFYR 250 260 270 280 290 300 TRDTRFGVAT AKVLQGKELS YNNMNDADAA FELVAEFDPQ RTAAVALIKH ANPCGVAEGQ 310 320 330 340 350 360 SLKEAYLKAL MCDSVSAFGG IVALNQPLDE ECAAEIVKLF TEVIIAPDAT EAACNIIAQK 370 380 390 400 410 420 KNLRLLITGG VPDPRCGGLT VKTLAGGVLV QSRDNAVVDD FDLQIVTKRA PSQDEMRDLQ 430 440 450 460 470 480 FAFRVVKHVK SNAIVYAKNS ATVGIGAGQM SRLDSARIAV HKAEDNAKRM GLTESLTRGS 490 500 510 520 530 VVASDAFFPF ADGLISAAEA GVTAVIQPGG SIRDQEVIEA ADARGLAMVF TGVRHFRH

References

[1]

Hendrix L., Mohamoud Y., Radune D., Shvartsbeyn A., Daugherty S., Dodson R., Durkin A.S., Harkins D., Huot H., Kothari S.P., Madupu R., Li J., Nelson W.C., Shrivastava S., Giglio M.G., Haft D., Selengut J., Fraser-Ligget C., Seshadri R. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000524 Genomic DNA. Translation: ABM45521.1.
RefSeq	YP_988412.1.
3D structure databases
SMR	A1UR09. Positions 16-538.
ModBase	Search...
Protein-protein interaction databases
STRING	A1UR09.
Genome annotation databases
GeneID	4684296.
GenomeReviews	Gene locus BARBAKC583_0074 in contig CP000524_GR.
KEGG	bbk:BARBAKC583_0074.
NMPDR	fig|360095.3.peg.1045.
TIGR	BARBAKC583_0074.
Phylogenomic databases
eggNOG	COG0138.
HOGENOM	HBG498048.
OMA	VVKHVKS.
Family and domain databases
HAMAP	MF_00139. PurH. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHase_bienz. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_BARBK

Accession

Primary (citable) accession number: A1UR09

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)