Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A1UPA5 (SYL_MYCSK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Mkms_5478
OrganismMycobacterium sp. (strain KMS) [Complete proteome] [HAMAP]
Taxonomic identifier189918 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length950 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence ABL94663.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 950950Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334777

Regions

Motif72 – 8312"HIGH" region HAMAP-Rule MF_00049
Motif722 – 7265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1UPA5 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: F3C2F0E3A9616CC6

FASTA950105,595
        10         20         30         40         50         60 
MTETPTAQPD RAADADTPQH RYTAELAGQI EGAWQQTWAV EGTFNVPNPV GELAPPDGTV 

        70         80         90        100        110        120 
PADKMFVQDM FPYPSGEGLH VGHPLGYIAT DVYARYYRMT GRNVLHALGF DAFGLPAEQY 

       130        140        150        160        170        180 
AVQTGTHPRT RTEANIVNFR RQLGRLGLGH DTRRSFSTTD VDFYTWTQWI FLQIYNAWFD 

       190        200        210        220        230        240 
RDANRARPIA ELIGEFESGV RTLDDGRPWS ELSAGERADV VDSYRLVYRA DSMVNWCPGL 

       250        260        270        280        290        300 
GTVLANEEVT ADGRSDRGNF PVFRKRLRQW MMRITAYSDR LLEDLEVLDW PDKVKTMQRN 

       310        320        330        340        350        360 
WIGRSTGASV QFGTDAGDIE VFTTRPDTLF GATYLVLAPE HPLVEQLAAE QWPDDVDGRW 

       370        380        390        400        410        420 
TFGATTPREA VAAYRASIAA KSDLERQENK TKTGAFLGAY ATNPANGQQV PIFIADYVLI 

       430        440        450        460        470        480 
GYGTGAIMAV PGHDQRDWEF AHEFGLPVVE VISGGDISEA AYAGDGLLVN SDYLDGLDVA 

       490        500        510        520        530        540 
AAKAAITDRL VADGRGRARV EYKLRDWLFA RQRYWGEPFP IVYDSDGRPH PLPESALPVE 

       550        560        570        580        590        600 
LPDVPDYSPV LFDPDDADSE PNPPLNKATD WVHVELDLGD GLQTYTRDTN VMPQWAGSSW 

       610        620        630        640        650        660 
YELRYTDPLN KEALCAKENE AYWMGPQPAE HGPDDPGGVD LYVGGVEHAV LHLLYSRFWH 

       670        680        690        700        710        720 
KVLYDLGHVS SREPYRRLVN QGYIQAFAYT DSRGSYVPAA EVVERDGKFW FEGAEVFQEF 

       730        740        750        760        770        780 
GKIGKSLKNS VSPDEICDNY GADTLRVYEM SMGPLEASRP WATKDVVGAH RFLQRVWRLV 

       790        800        810        820        830        840 
VDEQSGAVRV ANHEALDTDT LRALHRTVAG VSEDYAALRN NTAAAKLIEY TNHLTKEGVT 

       850        860        870        880        890        900 
ARAAIEPLVL MVAPLAPHLA EELWRRLGHD TSLAHGPFPV ADPQYLVTDT VEYPVQVNGK 

       910        920        930        940        950 
VRSRITVDAD AGKDTLEAAA LADEKVQAFL NGATPKKVIV VPGRLVNLVV 

« Hide

References

[1]"Complete sequence of chromosome of Mycobacterium sp. KMS."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KMS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000518 Genomic DNA. Translation: ABL94663.1. Different initiation.
RefSeqYP_941453.1. NC_008705.1.

3D structure databases

ProteinModelPortalA1UPA5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING189918.Mkms_5478.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL94663; ABL94663; Mkms_5478.
GeneID4613162.
KEGGmkm:Mkms_5478.
PATRIC18110034. VBIMycSp70743_6017.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycMSP189918:GH4X-5535-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_MYCSK
AccessionPrimary (citable) accession number: A1UPA5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries