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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Mycobacterium sp. (strain KMS)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei725 – 7251ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSP189918:GH4X-5535-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Mkms_5478
OrganismiMycobacterium sp. (strain KMS)
Taxonomic identifieri189918 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000638 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 950950Leucine--tRNA ligasePRO_0000334777Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi189918.Mkms_5478.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi72 – 8312"HIGH" regionAdd
BLAST
Motifi722 – 7265"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1UPA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTETPTAQPD RAADADTPQH RYTAELAGQI EGAWQQTWAV EGTFNVPNPV
60 70 80 90 100
GELAPPDGTV PADKMFVQDM FPYPSGEGLH VGHPLGYIAT DVYARYYRMT
110 120 130 140 150
GRNVLHALGF DAFGLPAEQY AVQTGTHPRT RTEANIVNFR RQLGRLGLGH
160 170 180 190 200
DTRRSFSTTD VDFYTWTQWI FLQIYNAWFD RDANRARPIA ELIGEFESGV
210 220 230 240 250
RTLDDGRPWS ELSAGERADV VDSYRLVYRA DSMVNWCPGL GTVLANEEVT
260 270 280 290 300
ADGRSDRGNF PVFRKRLRQW MMRITAYSDR LLEDLEVLDW PDKVKTMQRN
310 320 330 340 350
WIGRSTGASV QFGTDAGDIE VFTTRPDTLF GATYLVLAPE HPLVEQLAAE
360 370 380 390 400
QWPDDVDGRW TFGATTPREA VAAYRASIAA KSDLERQENK TKTGAFLGAY
410 420 430 440 450
ATNPANGQQV PIFIADYVLI GYGTGAIMAV PGHDQRDWEF AHEFGLPVVE
460 470 480 490 500
VISGGDISEA AYAGDGLLVN SDYLDGLDVA AAKAAITDRL VADGRGRARV
510 520 530 540 550
EYKLRDWLFA RQRYWGEPFP IVYDSDGRPH PLPESALPVE LPDVPDYSPV
560 570 580 590 600
LFDPDDADSE PNPPLNKATD WVHVELDLGD GLQTYTRDTN VMPQWAGSSW
610 620 630 640 650
YELRYTDPLN KEALCAKENE AYWMGPQPAE HGPDDPGGVD LYVGGVEHAV
660 670 680 690 700
LHLLYSRFWH KVLYDLGHVS SREPYRRLVN QGYIQAFAYT DSRGSYVPAA
710 720 730 740 750
EVVERDGKFW FEGAEVFQEF GKIGKSLKNS VSPDEICDNY GADTLRVYEM
760 770 780 790 800
SMGPLEASRP WATKDVVGAH RFLQRVWRLV VDEQSGAVRV ANHEALDTDT
810 820 830 840 850
LRALHRTVAG VSEDYAALRN NTAAAKLIEY TNHLTKEGVT ARAAIEPLVL
860 870 880 890 900
MVAPLAPHLA EELWRRLGHD TSLAHGPFPV ADPQYLVTDT VEYPVQVNGK
910 920 930 940 950
VRSRITVDAD AGKDTLEAAA LADEKVQAFL NGATPKKVIV VPGRLVNLVV
Length:950
Mass (Da):105,595
Last modified:May 20, 2008 - v2
Checksum:iF3C2F0E3A9616CC6
GO

Sequence cautioni

The sequence ABL94663.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA. Translation: ABL94663.1. Different initiation.
RefSeqiYP_941453.1. NC_008705.1.

Genome annotation databases

EnsemblBacteriaiABL94663; ABL94663; Mkms_5478.
KEGGimkm:Mkms_5478.
PATRICi18110034. VBIMycSp70743_6017.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA. Translation: ABL94663.1. Different initiation.
RefSeqiYP_941453.1. NC_008705.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi189918.Mkms_5478.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL94663; ABL94663; Mkms_5478.
KEGGimkm:Mkms_5478.
PATRICi18110034. VBIMycSp70743_6017.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciMSP189918:GH4X-5535-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KMS.

Entry informationi

Entry nameiSYL_MYCSK
AccessioniPrimary (citable) accession number: A1UPA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: April 1, 2015
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.