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Protein

Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase

Gene

Mkms_5144

Organism
Mycobacterium sp. (strain KMS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity).By similarity

Catalytic activityi

4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate.
Oxaloacetate = pyruvate + CO2.

Cofactori

a divalent metal cationBy similarityNote: Divalent metal cation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001SubstrateBy similarity
Metal bindingi101 – 1011Divalent metal cationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciMSP189918:GH4X-5200-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (EC:4.1.3.17)
Short name:
HMG aldolase
Alternative name(s):
Oxaloacetate decarboxylase (EC:4.1.1.3)
Short name:
OAA decarboxylase
Regulator of ribonuclease activity homolog
RraA-like protein
Gene namesi
Ordered Locus Names:Mkms_5144
OrganismiMycobacterium sp. (strain KMS)
Taxonomic identifieri189918 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000638 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 159159Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolasePRO_1000013851Add
BLAST

Interactioni

Subunit structurei

Homotrimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA1UNC5.
SMRiA1UNC5. Positions 3-157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni78 – 814Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class II aldolase/RraA-like family.Curated

Phylogenomic databases

HOGENOMiHOG000252803.
KOiK02553.
OMAiEDNTLVR.
OrthoDBiPOG091H03SM.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.

Sequencei

Sequence statusi: Complete.

A1UNC5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIEPRATAD LVDDIGPDVR SCDLQLRQFG RRPEFAGRVT TVRCFQDNAL
60 70 80 90 100
LKSVLSEPGD GGVLVIDGDG SLHTALVGDV IAALGRDNGW SGLIINGAVR
110 120 130 140 150
DASTLRTLDI GIKALGTNPR KSTKTGAGER DVPVDFGGVT FTPGDVAYSD

DDGIVVVTP
Length:159
Mass (Da):16,636
Last modified:February 6, 2007 - v1
Checksum:iFF1D55DF538B1100
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA. Translation: ABL94333.1.
RefSeqiWP_011562431.1. NC_008705.1.

Genome annotation databases

EnsemblBacteriaiABL94333; ABL94333; Mkms_5144.
KEGGimkm:Mkms_5144.
PATRICi18109348. VBIMycSp70743_5675.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA. Translation: ABL94333.1.
RefSeqiWP_011562431.1. NC_008705.1.

3D structure databases

ProteinModelPortaliA1UNC5.
SMRiA1UNC5. Positions 3-157.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL94333; ABL94333; Mkms_5144.
KEGGimkm:Mkms_5144.
PATRICi18109348. VBIMycSp70743_5675.

Phylogenomic databases

HOGENOMiHOG000252803.
KOiK02553.
OMAiEDNTLVR.
OrthoDBiPOG091H03SM.

Enzyme and pathway databases

BioCyciMSP189918:GH4X-5200-MONOMER.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRRAAH_MYCSK
AccessioniPrimary (citable) accession number: A1UNC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: September 7, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.