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Protein

Trehalose-6-phosphate synthase

Gene

otsA

Organism
Mycobacterium sp. (strain KMS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probably involved in the osmoprotection via the biosynthesis of trehalose and in the production of glycogen and alpha-glucan via the TreS-Pep2 branch involved in the biosynthesis of maltose-1-phosphate (M1P). Catalyzes the transfer of glucose from UDP-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate. Probably also able to use ADP-Glc, CDP-Glc, GDP-Glc and TDP-Glc as glucosyl donors.By similarity

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.By similarity
Nucleoside diphosphate-glucose + D-glucose 6-phosphate = trehalose 6-phosphate + nucleoside diphosphate.By similarity

Pathwayi: trehalose biosynthesis

This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22D-glucose 6-phosphateBy similarity1
Binding sitei94D-glucose 6-phosphateBy similarity1
Sitei103Involved in alpha anomer selectivityBy similarity1
Binding sitei148D-glucose 6-phosphateBy similarity1
Sitei173Involved in alpha anomer selectivityBy similarity1
Binding sitei290UDP-glucoseBy similarity1
Binding sitei295UDP-glucoseBy similarity1
Binding sitei328D-glucose 6-phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose-6-phosphate synthaseBy similarity (EC:2.4.1.15By similarity)
Short name:
TPSBy similarity
Alternative name(s):
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]By similarity
Osmoregulatory trehalose synthesis protein ABy similarity
Short name:
OtsABy similarity
Gene namesi
Name:otsABy similarity
Ordered Locus Names:Mkms_4697
OrganismiMycobacterium sp. (strain KMS)
Taxonomic identifieri189918 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000638 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003489121 – 489Trehalose-6-phosphate synthaseAdd BLAST489

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA1UM30.
SMRiA1UM30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni42 – 43UDP-glucose bindingBy similarity2
Regioni393 – 397UDP-glucose bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.By similarity

Phylogenomic databases

HOGENOMiHOG000191477.
KOiK00697.
OMAiIEFMPIH.
OrthoDBiPOG091H0ASR.

Family and domain databases

CDDicd03788. GT1_TPS. 1 hit.
InterProiView protein in InterPro
IPR001830. Glyco_trans_20.
PfamiView protein in Pfam
PF00982. Glyco_transf_20. 1 hit.

Sequencei

Sequence statusi: Complete.

A1UM30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEGDPGVG SGDSDFVVVA NRLPIDMERL PDGSTSFKRS PGGLVTALEP
60 70 80 90 100
LLRKRHGAWI GWAGIPDSAE DPIEDDGLQL YPVSLSADDV ADYYEGFSNA
110 120 130 140 150
TLWPLYHDLI VKPIYHRKWW DRYVEVNRRF AEATARAAAE GATVWVQDYQ
160 170 180 190 200
LQLVPKMLRM LRPDLTIGFF LHIPFPPVEL FMQMPWRTEI IEGLLGADLV
210 220 230 240 250
GFHLPGGAQN FLYLARRLTG ANTSRATVGV RSRFGEVQVG FRTVKVGAFP
260 270 280 290 300
ISIDSDELDG KARNRAVRQR AREIRNELGN PRKILLGVDR LDYTKGINVR
310 320 330 340 350
LEALSELLED GRVDSHDTVF VQLATPSRER VQSYIEMRED IERQVGHING
360 370 380 390 400
EFGDVGHPIV HYLHRPIPRD ELIAFFVAAD VMLVTPLRDG MNLVAKEYVA
410 420 430 440 450
CRSDLGGALV LSEFTGAAAE LRQAYLANPH HLEGVKDAIE AALNQDPEEG
460 470 480
RRRMRALRRQ VLAHDVDRWA RAFLDALADT RAGAKPVRD
Length:489
Mass (Da):54,726
Last modified:February 6, 2007 - v1
Checksum:iD8C558B2C21386D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA. Translation: ABL93888.1.
RefSeqiWP_011561986.1. NC_008705.1.

Genome annotation databases

EnsemblBacteriaiABL93888; ABL93888; Mkms_4697.
GeneIDi32421410.
KEGGimkm:Mkms_4697.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiOTSA_MYCSK
AccessioniPrimary (citable) accession number: A1UM30
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: February 6, 2007
Last modified: July 5, 2017
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families