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Protein

Trehalose-phosphate synthase

Gene

otsA

Organism
Mycobacterium sp. (strain KMS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6-phosphate and nucleoside diphosphate.By similarity

Catalytic activityi

Nucleoside diphosphate-glucose + D-glucose 6-phosphate = trehalose 6-phosphate + nucleoside diphosphate.

Pathwayi: trehalose biosynthesis

This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei22 – 221Glucose-6-phosphateBy similarity
Binding sitei94 – 941Glucose-6-phosphateBy similarity
Sitei103 – 1031Involved in alpha anomer selectivityBy similarity
Binding sitei148 – 1481Glucose-6-phosphateBy similarity
Sitei173 – 1731Involved in alpha anomer selectivityBy similarity
Binding sitei290 – 2901NDP-glucoseBy similarity
Binding sitei295 – 2951NDP-glucoseBy similarity
Binding sitei328 – 3281Glucose-6-phosphateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMSP189918:GH4X-4745-MONOMER.
UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose-phosphate synthase (EC:2.4.1.-)
Short name:
TPS
Alternative name(s):
Trehalose-6-phosphate synthase
Gene namesi
Name:otsA
Ordered Locus Names:Mkms_4697
OrganismiMycobacterium sp. (strain KMS)
Taxonomic identifieri189918 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000638 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Trehalose-phosphate synthasePRO_0000348912Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliA1UM30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni42 – 432NDP-glucose bindingBy similarity
Regioni393 – 3975NDP-glucose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.Curated

Phylogenomic databases

HOGENOMiHOG000191477.
KOiK00697.
OMAiPEWIGKI.
OrthoDBiEOG6CCH1M.

Family and domain databases

InterProiIPR001830. Glyco_trans_20.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1UM30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEGDPGVG SGDSDFVVVA NRLPIDMERL PDGSTSFKRS PGGLVTALEP
60 70 80 90 100
LLRKRHGAWI GWAGIPDSAE DPIEDDGLQL YPVSLSADDV ADYYEGFSNA
110 120 130 140 150
TLWPLYHDLI VKPIYHRKWW DRYVEVNRRF AEATARAAAE GATVWVQDYQ
160 170 180 190 200
LQLVPKMLRM LRPDLTIGFF LHIPFPPVEL FMQMPWRTEI IEGLLGADLV
210 220 230 240 250
GFHLPGGAQN FLYLARRLTG ANTSRATVGV RSRFGEVQVG FRTVKVGAFP
260 270 280 290 300
ISIDSDELDG KARNRAVRQR AREIRNELGN PRKILLGVDR LDYTKGINVR
310 320 330 340 350
LEALSELLED GRVDSHDTVF VQLATPSRER VQSYIEMRED IERQVGHING
360 370 380 390 400
EFGDVGHPIV HYLHRPIPRD ELIAFFVAAD VMLVTPLRDG MNLVAKEYVA
410 420 430 440 450
CRSDLGGALV LSEFTGAAAE LRQAYLANPH HLEGVKDAIE AALNQDPEEG
460 470 480
RRRMRALRRQ VLAHDVDRWA RAFLDALADT RAGAKPVRD
Length:489
Mass (Da):54,726
Last modified:February 6, 2007 - v1
Checksum:iD8C558B2C21386D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA. Translation: ABL93888.1.
RefSeqiWP_011561986.1. NC_008705.1.

Genome annotation databases

EnsemblBacteriaiABL93888; ABL93888; Mkms_4697.
KEGGimkm:Mkms_4697.
PATRICi18108442. VBIMycSp70743_5229.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA. Translation: ABL93888.1.
RefSeqiWP_011561986.1. NC_008705.1.

3D structure databases

ProteinModelPortaliA1UM30.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL93888; ABL93888; Mkms_4697.
KEGGimkm:Mkms_4697.
PATRICi18108442. VBIMycSp70743_5229.

Phylogenomic databases

HOGENOMiHOG000191477.
KOiK00697.
OMAiPEWIGKI.
OrthoDBiEOG6CCH1M.

Enzyme and pathway databases

UniPathwayiUPA00299.
BioCyciMSP189918:GH4X-4745-MONOMER.

Family and domain databases

InterProiIPR001830. Glyco_trans_20.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KMS.

Entry informationi

Entry nameiOTSA_MYCSK
AccessioniPrimary (citable) accession number: A1UM30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: February 6, 2007
Last modified: December 9, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.