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A1UJW9 (GLGB_MYCSK) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Mkms_3935
OrganismMycobacterium sp. (strain KMS) [Complete proteome] [HAMAP]
Taxonomic identifier189918 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length759 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7597591,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000044984

Sites

Active site4221Nucleophile By similarity
Active site4751Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
A1UJW9 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: F96AA2E4D060156C

FASTA75984,965
        10         20         30         40         50         60 
MAKTKGLPKD TAVTPSPHLR PHTADLNRLL AGEHHDPHSI LGAHEYDDHT VIRAYRPHAT 

        70         80         90        100        110        120 
EVAAVVGGER HVFTHLEAGV FAVTLPFTGL IDYRLEVGYD HGGDQPHIHH TADAYRFLPT 

       130        140        150        160        170        180 
LGEMDLHLFS EGRHERLWEV LGAHPRTFET PDGVVEGVSF AVWAPNANGV QLIGDFNHWD 

       190        200        210        220        230        240 
GNEAQLRVLG STGVWELFWP DFPVDGLYKF RIHGADGVVS ERADPMAFAT EVPPQTASRV 

       250        260        270        280        290        300 
TTSSYTWNDD AWMTQRAAQN PVFEPMSTLE VHLMSWRPGL SYVELADQLT EYIVEHGFTH 

       310        320        330        340        350        360 
VEMLPVAEHP FGGSWGYQVT SYYAPTSRLG TPDEFRYLVD RLHQAGIGVI VDWVPAHFPK 

       370        380        390        400        410        420 
DAWALGRFDG TALYEHADPR RGEQLDWGTY VFDFGRAEVR NFLVANALYW LQEFHVDGLR 

       430        440        450        460        470        480 
VDAVASMLYL DYSRPEGGWT PNIYGGRENL EAVQFLQEMN ATVHKASPGI VTIAEESTSW 

       490        500        510        520        530        540 
PGVTRPTNLG GLGFSMKWNM GWMNDTLAFI SRDPIHRSYH HHEMTFSMLY AFSENYVLPI 

       550        560        570        580        590        600 
SHDEVVHGKG TLWGRMLGDD HRKAAGVRQL LAYQWAHPGK QLLFQGQEFG QRAEWSEERG 

       610        620        630        640        650        660 
VDWYQLDENS YSGGILRMIS DMNGIYTSHR ALWSHDTSPE GYSWIDANDS TNNVLSFLRY 

       670        680        690        700        710        720 
GDDGSVLACV FNFSGSEHSQ YRLGLPHAGT WREVLNTDAA DYNGAGIGNY GAVQATDEPW 

       730        740        750 
HGRPASAVMV LPPLSALWFE PVAAEAPVVQ EPPTAPPLS 

« Hide

References

[1]"Complete sequence of chromosome of Mycobacterium sp. KMS."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KMS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000518 Genomic DNA. Translation: ABL93127.1.
RefSeqYP_939917.1. NC_008705.1.

3D structure databases

ProteinModelPortalA1UJW9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING189918.Mkms_3935.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL93127; ABL93127; Mkms_3935.
GeneID4611871.
KEGGmkm:Mkms_3935.
PATRIC18106872. VBIMycSp70743_4457.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAVACVFNF.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycMSP189918:GH4X-3970-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_MYCSK
AccessionPrimary (citable) accession number: A1UJW9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries