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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Mycobacterium sp. (strain KMS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei422 – 4221NucleophileUniRule annotation
Active sitei475 – 4751Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciMSP189918:GH4X-3970-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Mkms_3935
OrganismiMycobacterium sp. (strain KMS)
Taxonomic identifieri189918 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000638: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7597591,4-alpha-glucan branching enzyme GlgBPRO_1000044984Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi189918.Mkms_3935.

Structurei

3D structure databases

ProteinModelPortaliA1UJW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDATEVWV.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

A1UJW9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKTKGLPKD TAVTPSPHLR PHTADLNRLL AGEHHDPHSI LGAHEYDDHT
60 70 80 90 100
VIRAYRPHAT EVAAVVGGER HVFTHLEAGV FAVTLPFTGL IDYRLEVGYD
110 120 130 140 150
HGGDQPHIHH TADAYRFLPT LGEMDLHLFS EGRHERLWEV LGAHPRTFET
160 170 180 190 200
PDGVVEGVSF AVWAPNANGV QLIGDFNHWD GNEAQLRVLG STGVWELFWP
210 220 230 240 250
DFPVDGLYKF RIHGADGVVS ERADPMAFAT EVPPQTASRV TTSSYTWNDD
260 270 280 290 300
AWMTQRAAQN PVFEPMSTLE VHLMSWRPGL SYVELADQLT EYIVEHGFTH
310 320 330 340 350
VEMLPVAEHP FGGSWGYQVT SYYAPTSRLG TPDEFRYLVD RLHQAGIGVI
360 370 380 390 400
VDWVPAHFPK DAWALGRFDG TALYEHADPR RGEQLDWGTY VFDFGRAEVR
410 420 430 440 450
NFLVANALYW LQEFHVDGLR VDAVASMLYL DYSRPEGGWT PNIYGGRENL
460 470 480 490 500
EAVQFLQEMN ATVHKASPGI VTIAEESTSW PGVTRPTNLG GLGFSMKWNM
510 520 530 540 550
GWMNDTLAFI SRDPIHRSYH HHEMTFSMLY AFSENYVLPI SHDEVVHGKG
560 570 580 590 600
TLWGRMLGDD HRKAAGVRQL LAYQWAHPGK QLLFQGQEFG QRAEWSEERG
610 620 630 640 650
VDWYQLDENS YSGGILRMIS DMNGIYTSHR ALWSHDTSPE GYSWIDANDS
660 670 680 690 700
TNNVLSFLRY GDDGSVLACV FNFSGSEHSQ YRLGLPHAGT WREVLNTDAA
710 720 730 740 750
DYNGAGIGNY GAVQATDEPW HGRPASAVMV LPPLSALWFE PVAAEAPVVQ

EPPTAPPLS
Length:759
Mass (Da):84,965
Last modified:February 6, 2007 - v1
Checksum:iF96AA2E4D060156C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA. Translation: ABL93127.1.
RefSeqiYP_939917.1. NC_008705.1.

Genome annotation databases

EnsemblBacteriaiABL93127; ABL93127; Mkms_3935.
GeneIDi4611871.
KEGGimkm:Mkms_3935.
PATRICi18106872. VBIMycSp70743_4457.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA. Translation: ABL93127.1.
RefSeqiYP_939917.1. NC_008705.1.

3D structure databases

ProteinModelPortaliA1UJW9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi189918.Mkms_3935.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL93127; ABL93127; Mkms_3935.
GeneIDi4611871.
KEGGimkm:Mkms_3935.
PATRICi18106872. VBIMycSp70743_4457.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDATEVWV.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciMSP189918:GH4X-3970-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KMS.

Entry informationi

Entry nameiGLGB_MYCSK
AccessioniPrimary (citable) accession number: A1UJW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: January 7, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.