Reviewed, UniProtKB/Swiss-Prot A1UJP3 (MHPB_MYCSK)

Names and origin

Protein names

Recommended name:
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
    EC=1.13.11.16

Gene names

Name:	mhpB
Ordered Locus Names:	Mkms_3858

Organism

Mycobacterium sp. (strain KMS) [Complete proteome] [HAMAP]

Taxonomic identifier

189918 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity.
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP MF_01653 2,3-dihydroxicinnamic acid + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP MF_01653
Cofactor	Fe2+ ion By similarity.
Pathway	Aromatic compound metabolism; 3-phenylpropionic acid degradation. HAMAP MF_01653
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydroxy-phenylpropionate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP 3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase HAMAP MF_01653		PRO_0000337657
Sites
	Active site	116	1	Proton donor By similarity
	Active site	180	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1UJP3-1 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: 2FE96B1CB6B9219F	FASTA	313	33,047
	10 20 30 40 50 60 MAQIALCCTS HSPLLNLPGP SRELLDDIGS ALAVARDFVT EFDPDLVVTF SPDHYNGFFY 70 80 90 100 110 120 KVMPPFCVGT SAQGVGDYGT HAGPLDVPED LANELATAVL EAGVDVAISA SMDVDHGTVQ 130 140 150 160 170 180 PLQNLFGDAM ARPVIPVFIN SVATPLGPLR RTRALGTAIG RYLATLDKRV LVIGSGGLSH 190 200 210 220 230 240 DPPVPTLATA PPAALDRIVH GAPMSTEQRM ARQSAVIDAA HAFAHGESPL QPLNPAWDAT 250 260 270 280 290 300 FLEILDEGWL SDLDGWSNAF IAREGGNSAH EIRTWVAAFA ALAAGGDYRT GLRFYRAAPE 310 LIAGFAIRTA VLA

References

[1]

"Complete sequence of chromosome of Mycobacterium sp. KMS." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000518 Genomic DNA. Translation: ABL93051.1. Different initiation.
RefSeq	YP_939841.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4611793.
GenomeReviews	Gene locus Mkms_3858 in contig CP000518_GR.
KEGG	mkm:Mkms_3858.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01653. [Tree]
InterPro	IPR004183. Xdiol_dOase_3B. [Graphical view]
Pfam	PF02900. LigB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MHPB_MYCSK

Accession

Primary (citable) accession number: A1UJP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	June 16, 2009
This is version 18 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)