A1UIZ7 (PROA_MYCSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Mkms_3611

Organism

Mycobacterium sp. (strain KMS) [Complete proteome] [HAMAP]

Taxonomic identifier

189918 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000049966

Sequences

	Sequence		Length	Mass (Da)
	A1UIZ7 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: BB8F57C4F0A105AA	FASTA	415	43,482
	10 20 30 40 50 60 MSVHAPAAPD LRTEVHDAAR RARVASRTLA TLSTETKNRA LRAAADRVLM DAHLIIAGNE 70 80 90 100 110 120 RDLEKARAAG TPDAMLDRLA LNPQRIDGVA AGLRQVASLP DPVGEVLRGN TLVNGLQLRQ 130 140 150 160 170 180 QRVPLGVVGI VYEGRPNVTV DAFGLTLKSG NAVLLRGSSS AAHSNAALVD SLRAALVAEG 190 200 210 220 230 240 LDANAVQLLP SHDRASVTHL IQARGLVDVV IPRGGAGLID AVVRDAQVPT IETGVGNCHV 250 260 270 280 290 300 YVHSAADLDM AETILLNAKT RRPSVCNAAE SVLIDAAIAE EAVPRLTKAL QDAGVTVHAD 310 320 330 340 350 360 PTEEELRAEF LSMDIALAVV DGVDAAIAHV NEYGSGHTEA IVTADLAAAQ RFTERVDAAA 370 380 390 400 410 VMVNASTAFT DGEQFGFGAE IGISTQKLHA RGPMGLPELT STKWIVWGDG HTRPA

References

[1]

"Complete sequence of chromosome of Mycobacterium sp. KMS." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KMS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000518 Genomic DNA. Translation: ABL92805.1.
RefSeq	YP_939595.1. NC_008705.1.
3D structure databases
ProteinModelPortal	A1UIZ7.
ModBase	Search...
Protein-protein interaction databases
STRING	A1UIZ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000057000; EBMYCP00000055224; EBMYCG00000056995.
GeneID	4611541.
GenomeReviews	Gene locus Mkms_3611 in contig CP000518_GR.
KEGG	mkm:Mkms_3611.
PATRIC	18106204. VBIMycSp70743_4129.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
GeneTree	EBGT00050000015663.
HOGENOM	HBG318080.
OMA	QYPAACN.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	MSP189918:MKMS_3611-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_MYCSK

Accession

Primary (citable) accession number: A1UIZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program