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A1UHS7

- PSB_MYCSK

UniProt

A1UHS7 - PSB_MYCSK

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Protein

Proteasome subunit beta

Gene

prcB

Organism
Mycobacterium sp. (strain KMS)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.UniRule annotation

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.UniRule annotation

Enzyme regulationi

The formation of the proteasomal ATPase ARC-20S proteasome complex, likely via the docking of the C-termini of ARC into the intersubunit pockets in the alpha-rings, may trigger opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661NucleophileUniRule annotation

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. modification-dependent protein catabolic process Source: UniProtKB-HAMAP
  2. proteasomal protein catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

BioCyciMSP189918:GH4X-3214-MONOMER.
UniPathwayiUPA00997.

Protein family/group databases

MEROPSiT01.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit betaUniRule annotation (EC:3.4.25.1UniRule annotation)
Alternative name(s):
20S proteasome beta subunitUniRule annotation
Proteasome core protein PrcBUniRule annotation
Gene namesi
Name:prcBUniRule annotation
Ordered Locus Names:Mkms_3191
OrganismiMycobacterium sp. (strain KMS)
Taxonomic identifieri189918 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000638: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
  2. proteasome core complex, beta-subunit complex Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 6565Removed in mature form; by autocatalysisUniRule annotationPRO_0000397542Add
BLAST
Chaini66 – 304239Proteasome subunit betaPRO_0000397543Add
BLAST

Keywords - PTMi

Autocatalytic cleavage, Zymogen

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped by the proteasome-associated ATPase, ARC.UniRule annotation

Protein-protein interaction databases

STRINGi189918.Mkms_3191.

Structurei

3D structure databases

ProteinModelPortaliA1UHS7.
SMRiA1UHS7. Positions 32-287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000245308.
KOiK03433.
OMAiFQVELEH.
OrthoDBiEOG6XM79W.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_02113_B. Proteasome_B_B.
InterProiIPR029055. Ntn_hydrolases_N.
IPR022483. Pept_T1A_Psome_suB_actinobac.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03690. 20S_bact_beta. 1 hit.
PROSITEiPS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1UHS7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTWPHFEQLA FPDLSRHSSH STTRGVPSVP MDLSSFSDML RRQAPHLLPF
60 70 80 90 100
RGDASLTPTD AVPHGTTIVA LKFPGGVVMA GDRRATQGNM IASRDVQKVY
110 120 130 140 150
ITDDYTATGI AGTAAIAVEF ARLYAVELEH YEKLEGVALT FAGKVNRLAT
160 170 180 190 200
MVRGNLGAAL QGFVALPLLA GFDLDDPDPQ AAGRIVSFDA AGGHNLEEEG
210 220 230 240 250
FQSVGSGSIF AKSSMKKLYH QVTDADSALR VAVEALYDAA DDDSATGGPD
260 270 280 290 300
LVRGIFPTAV LITADGAEEV TQERIAGLAR EVIQNRSRAD TFGPDAHAPR

GTDS
Length:304
Mass (Da):32,266
Last modified:February 6, 2007 - v1
Checksum:iB52436CB43F2A9F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA. Translation: ABL92385.1.
RefSeqiYP_939175.1. NC_008705.1.

Genome annotation databases

EnsemblBacteriaiABL92385; ABL92385; Mkms_3191.
GeneIDi4611115.
KEGGimkm:Mkms_3191.
PATRICi18105366. VBIMycSp70743_3714.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA. Translation: ABL92385.1 .
RefSeqi YP_939175.1. NC_008705.1.

3D structure databases

ProteinModelPortali A1UHS7.
SMRi A1UHS7. Positions 32-287.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 189918.Mkms_3191.

Protein family/group databases

MEROPSi T01.005.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABL92385 ; ABL92385 ; Mkms_3191 .
GeneIDi 4611115.
KEGGi mkm:Mkms_3191.
PATRICi 18105366. VBIMycSp70743_3714.

Phylogenomic databases

eggNOGi COG0638.
HOGENOMi HOG000245308.
KOi K03433.
OMAi FQVELEH.
OrthoDBi EOG6XM79W.

Enzyme and pathway databases

UniPathwayi UPA00997 .
BioCyci MSP189918:GH4X-3214-MONOMER.

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
HAMAPi MF_02113_B. Proteasome_B_B.
InterProi IPR029055. Ntn_hydrolases_N.
IPR022483. Pept_T1A_Psome_suB_actinobac.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view ]
Pfami PF00227. Proteasome. 1 hit.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR03690. 20S_bact_beta. 1 hit.
PROSITEi PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KMS.

Entry informationi

Entry nameiPSB_MYCSK
AccessioniPrimary (citable) accession number: A1UHS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: February 6, 2007
Last modified: October 29, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3