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Protein

6,7-dimethyl-8-ribityllumazine synthase

Gene

ribH

Organism
Mycobacterium sp. (strain KMS)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.UniRule annotation

Catalytic activityi

1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H2O + phosphate.UniRule annotation

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 6,7-dimethyl-8-ribityllumazine synthase (ribH)
  2. no protein annotated in this organism
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei275-amino-6-(D-ribitylamino)uracilUniRule annotation1
Active sitei89Proton donorUniRule annotation1
Binding sitei1145-amino-6-(D-ribitylamino)uracil; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei1281-deoxy-L-glycero-tetrulose 4-phosphateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processRiboflavin biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00275; UER00404

Names & Taxonomyi

Protein namesi
Recommended name:
6,7-dimethyl-8-ribityllumazine synthaseUniRule annotation (EC:2.5.1.78UniRule annotation)
Short name:
DMRL synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lumazine synthaseUniRule annotation
Gene namesi
Name:ribHUniRule annotation
Ordered Locus Names:Mkms_2438
OrganismiMycobacterium sp. (strain KMS)
Taxonomic identifieri189918 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000404561 – 1606,7-dimethyl-8-ribityllumazine synthaseAdd BLAST160

Interactioni

Subunit structurei

Homopentamer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA1UFM8
SMRiA1UFM8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni59 – 615-amino-6-(D-ribitylamino)uracil bindingUniRule annotation3
Regioni81 – 835-amino-6-(D-ribitylamino)uracil bindingUniRule annotation3
Regioni86 – 871-deoxy-L-glycero-tetrulose 4-phosphate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the DMRL synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000229253
KOiK00794
OMAiHGNKGTE
OrthoDBiPOG091H021R

Family and domain databases

CDDicd09209 Lumazine_synthase-I, 1 hit
Gene3Di3.40.50.960, 1 hit
HAMAPiMF_00178 Lumazine_synth, 1 hit
InterProiView protein in InterPro
IPR034964 LS
IPR002180 LS/RS
IPR036467 LS/RS_sf
PANTHERiPTHR21058 PTHR21058, 1 hit
PfamiView protein in Pfam
PF00885 DMRL_synthase, 1 hit
SUPFAMiSSF52121 SSF52121, 1 hit
TIGRFAMsiTIGR00114 lumazine-synth, 1 hit

Sequencei

Sequence statusi: Complete.

A1UFM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGAGVPDL PQIDASGMKV GIVASTWHST ICDALLDGAL KVTESANVSE
60 70 80 90 100
PTVVRVLGAI EIPVVAQALA ADHEAVIALG VVIRGQTPHF DYVCDAVTQG
110 120 130 140 150
LTRVSLDAST PVANGVLTTN TEEQALDRAG LPGSAEDKGA QAAAAALSTA
160
LTLQGLRGRS
Length:160
Mass (Da):16,110
Last modified:February 6, 2007 - v1
Checksum:i9E1B9A2F4015006A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA Translation: ABL91636.1
RefSeqiWP_011559788.1, NC_008705.1

Genome annotation databases

EnsemblBacteriaiABL91636; ABL91636; Mkms_2438
GeneIDi32420629
KEGGimkm:Mkms_2438

Similar proteinsi

Entry informationi

Entry nameiRISB_MYCSK
AccessioniPrimary (citable) accession number: A1UFM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: March 28, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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