A1UF44 (DXS_MYCSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	Mkms_2254

Organism

Mycobacterium sp. (strain KMS) [Complete proteome] [HAMAP]

Taxonomic identifier

189918 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 638	638	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000019043

Sequences

	Sequence		Length	Mass (Da)
	A1UF44 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 87B204803A8379B2	FASTA	638	68,120
	10 20 30 40 50 60 MLEQIRGPAD LQHLSQSALS ELAGEIRQFL IHKVAATGGH LGPNLGVVEL TLALHRVFDS 70 80 90 100 110 120 PHDPLIFDTG HQAYVHKMLT GRSHEFDSLR KKDGLSGYPS RSESEHDWVE SSHASAALSY 130 140 150 160 170 180 ADGLAKAFEL TGHRNRHVVA VVGDGALTGG MCWEALNNIA AARRPVVIVV NDNGRSYAPT 190 200 210 220 230 240 IGGFADHLAA LRLQPGYERV LEEGRKAVRG LPVIGEFCYQ CMHSVKAGIK DALSPQVMFT 250 260 270 280 290 300 DLGLKYVGPI DGHDEHAVES ALRHARGFNA PVIVHVVTRK GMGYAPAEND EAEQMHACGV 310 320 330 340 350 360 IDVATGRATK VAAPGWTSSF SEALIDYGAK RRDIVAITAA MPGPTGLSAF RDRFPDRFFD 370 380 390 400 410 420 VGIAEQHAMT SAAGLAMGGL HPVVAIYSTF LNRAFDQLMM DVALHKLPVT LVLDRSGVTG 430 440 450 460 470 480 PDGASHNGMW DLSVLGIVPG MRVAAPRDGA RLREELGEAL DVNDAPTAIR FPKGDVGEDI 490 500 510 520 530 540 PAVRRHRGVD VLAEPADGLS DDVLLVAVGP FASMALTVAE RLRKQGIGVT VVDPRWVLPV 550 560 570 580 590 600 PEVLTEFAAA HKLVVTVEDN GLHGGIGSSV SAALRHAEVD VPCRDVGLPQ QFFDHASRGE 610 620 630 VLADVGVTDR NISRQITGWV AALGATPADA DEVSERLD

References

[1]

"Complete sequence of chromosome of Mycobacterium sp. KMS." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KMS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000518 Genomic DNA. Translation: ABL91452.1.
RefSeq	YP_938242.1. NC_008705.1.
3D structure databases
ProteinModelPortal	A1UF44.
ModBase	Search...
Protein-protein interaction databases
STRING	A1UF44.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000058179; EBMYCP00000056403; EBMYCG00000058174.
GeneID	4613431.
GenomeReviews	Gene locus Mkms_2254 in contig CP000518_GR.
KEGG	mkm:Mkms_2254.
PATRIC	18103439. VBIMycSp70743_2761.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
GeneTree	EBGT00050000016269.
HOGENOM	HBG571647.
OMA	QYDISYM.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	MSP189918:MKMS_2254-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR011766. TPP_enzyme-bd_C. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_MYCSK

Accession

Primary (citable) accession number: A1UF44

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program