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A1UEZ9

- DAPF_MYCSK

UniProt

A1UEZ9 - DAPF_MYCSK

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Mycobacterium sp. (strain KMS)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei11 – 111SubstrateUniRule annotation
    Binding sitei44 – 441SubstrateUniRule annotation
    Binding sitei78 – 781SubstrateUniRule annotation
    Active sitei87 – 871Proton donor/acceptorUniRule annotation
    Binding sitei166 – 1661SubstrateUniRule annotation
    Sitei168 – 1681Important for catalytic activityUniRule annotation
    Binding sitei202 – 2021SubstrateUniRule annotation
    Sitei220 – 2201Important for catalytic activityUniRule annotation
    Active sitei229 – 2291Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciMSP189918:GH4X-2222-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:Mkms_2209
    OrganismiMycobacterium sp. (strain KMS)
    Taxonomic identifieri189918 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000638: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 293293Diaminopimelate epimerasePRO_1000011911Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi87 ↔ 229

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi189918.Mkms_2209.

    Structurei

    3D structure databases

    ProteinModelPortaliA1UEZ9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni87 – 893Substrate bindingUniRule annotation
    Regioni220 – 2212Substrate bindingUniRule annotation
    Regioni230 – 2312Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220467.
    KOiK01778.
    OMAiITIEMGT.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1UEZ9-1 [UniParc]FASTAAdd to Basket

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    MQFAKGHGTQ NDFVLLPDLD ARLALTPAVV SALCDRRRGL GADGVLRVTT    50
    AKAALSAGVF ERLPEGVGAG DWYMDYRNAD GSIAQMCGNG VRVFAHYLRA 100
    ADLESRDEFV VGSLAGPRPV VLHGFDLAHR SRAEVTVEMG KVNLLGSGSA 150
    VVGGRRFTGL GIDVGNPHLA CVDTTLTEAE LAALDVAAPV DFDPAQFPDG 200
    VNVEVLTALR DGAVSMRVHE RGVGETRSCG TGTVAAAVAA LAQEGAAVGT 250
    LDVRIPGGVV TVSVTDTTSF LRGPSELVAT GELAGEWWQS HQR 293
    Length:293
    Mass (Da):30,530
    Last modified:February 6, 2007 - v1
    Checksum:i25A9C82578F1765A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000518 Genomic DNA. Translation: ABL91407.1.
    RefSeqiYP_938197.1. NC_008705.1.

    Genome annotation databases

    EnsemblBacteriaiABL91407; ABL91407; Mkms_2209.
    GeneIDi4613386.
    KEGGimkm:Mkms_2209.
    PATRICi18103347. VBIMycSp70743_2715.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000518 Genomic DNA. Translation: ABL91407.1 .
    RefSeqi YP_938197.1. NC_008705.1.

    3D structure databases

    ProteinModelPortali A1UEZ9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 189918.Mkms_2209.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABL91407 ; ABL91407 ; Mkms_2209 .
    GeneIDi 4613386.
    KEGGi mkm:Mkms_2209.
    PATRICi 18103347. VBIMycSp70743_2715.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220467.
    KOi K01778.
    OMAi ITIEMGT.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci MSP189918:GH4X-2222-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KMS.

    Entry informationi

    Entry nameiDAPF_MYCSK
    AccessioniPrimary (citable) accession number: A1UEZ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3