A1UER2 (SYP_MYCSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Mkms_2122

Organism

Mycobacterium sp. (strain KMS) [Complete proteome] [HAMAP]

Taxonomic identifier

189918 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 584	584	Proline--tRNA ligase HAMAP MF_01569		PRO_0000288351

Sequences

	Sequence		Length	Mass (Da)
	A1UER2 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: B32538BEBA5AB2C4	FASTA	584	63,945
	10 20 30 40 50 60 MITRMSELFL RTLRDDPADA EVPSHKLLIR AGYVRPVGPG LYTWLPLGLR VFRKIEQIVR 70 80 90 100 110 120 DEMTAIGGQE ILFPALLPRA PYETTNRWTE YGDTLFRLKD RRDNDYLLGP THEELFTLTV 130 140 150 160 170 180 KGEYSSYKDF PLILFQIQTK YRDEARPRAG ILRGREFVMK DSYSFDVDDD GLKTAYHLHR 190 200 210 220 230 240 EAYQRIFARL GVHYVIVSAV SGAMGGSASE EFLAESEVGE DTFVRCLQSG YAANVEAVLT 250 260 270 280 290 300 RVPEPLPIEG QPEAVVYDTP DAPTIATLVD WANGADLPNF AGRAVTAADT LKNVLVKVRE 310 320 330 340 350 360 PGGEWELLAV GVPGDREVDD KRLGAALEPA EYALLDEADF ARHPFLVKGY VGPKALLDNG 370 380 390 400 410 420 VRYLVDPRVV DGTAWITGAD APNKHVVGLV AGRDFVADGT IEAAEVRDGD PSPDGAGPLV 430 440 450 460 470 480 SARGIEIGHI FQLGRKYTEA FSADVLGEDG KPVRLTMGSY GIGVSRLVAV IAEQQHDELG 490 500 510 520 530 540 LRWPAAVAPF DVHVVIANKD DGARTGATEL AGELDRLGLE VLLDDRKSSP GVKFKDAELL 550 560 570 580 GVPWIVVVGR GWGDGVVELR DRFSGEKREI GVDDAATEIL ATVR

References

[1]

"Complete sequence of chromosome of Mycobacterium sp. KMS." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KMS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000518 Genomic DNA. Translation: ABL91320.1.
RefSeq	YP_938110.1. NC_008705.1.
3D structure databases
ProteinModelPortal	A1UER2.
ModBase	Search...
Protein-protein interaction databases
STRING	A1UER2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000053391; EBMYCP00000051615; EBMYCG00000053386.
GeneID	4613481.
GenomeReviews	Gene locus Mkms_2122 in contig CP000518_GR.
KEGG	mkm:Mkms_2122.
PATRIC	18103165. VBIMycSp70743_2624.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
GeneTree	EBGT00050000016802.
HOGENOM	HBG403504.
OMA	IQPAELW.
ProtClustDB	PRK09194.
Enzyme and pathway databases
BioCyc	MSP189918:MKMS_2122-MONOMER.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_MYCSK

Accession

Primary (citable) accession number: A1UER2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program