Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A1U995 (PCKG_MYCSK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]

Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Gene names
Name:pckG
Ordered Locus Names:Mkms_0185
OrganismMycobacterium sp. (strain KMS) [Complete proteome] [HAMAP]
Taxonomic identifier189918 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Protein attributes

Sequence length609 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP-Rule MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00452

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00452

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 609609Phosphoenolpyruvate carboxykinase [GTP] HAMAP-Rule MF_00452
PRO_1000060294

Regions

Nucleotide binding272 – 2776GTP By similarity
Nucleotide binding515 – 5184GTP By similarity
Region387 – 3893Substrate binding By similarity

Sites

Active site2731 By similarity
Metal binding2291Manganese By similarity
Metal binding2491Manganese; via tele nitrogen By similarity
Metal binding2961Manganese By similarity
Binding site811Substrate By similarity
Binding site2221Substrate; via amide nitrogen By similarity
Binding site2291Substrate By similarity
Binding site2711Substrate By similarity
Binding site3891GTP By similarity
Binding site4201GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1U995 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 9F7D9E191F37FA54

FASTA60967,822
        10         20         30         40         50         60 
MTSATIPGLD TAPTEHEGLL AWVREVAELT QPDRVVFTDG SEEECARLTE QLCEAGTFQK 

        70         80         90        100        110        120 
LNEEKKPNSY LALSDPSDVA RVESRTYICS EREIDAGPTN NWMDPAEMRG IMTDLYRGSM 

       130        140        150        160        170        180 
RGRTMYVVPF CMGPLEAEDP KLGVEITDSE YVVVSMRTMT RMGQAALDKM GTDGFFVKAL 

       190        200        210        220        230        240 
HSLGAPLEPG EKDVPWPCND TKYITHFPET REIWSYGSGY GGNALLGKKC YSLRIASAMA 

       250        260        270        280        290        300 
HDEGWLAEHM LILKLISPEN KAYFIAAAFP SACGKTNLAM LQPTIPGWRA ETVGDDIAWM 

       310        320        330        340        350        360 
RFGKDGRLYA VNPEFGFFGV APGTNWSSNP NAMKTIEAGN TVFTNVAKTD DGDVWWEGLE 

       370        380        390        400        410        420 
GEPDHLIDWK GNDYILRETE TKAAHPNSRY CTPISQCPTL APEWDDPQGV PISAILFGGR 

       430        440        450        460        470        480 
RKTTVPLITQ ARDWQHGVFI GATLGSEQTA AAEGKVGTVR RDPMAMLPFL GYNVGDYFQH 

       490        500        510        520        530        540 
WIDIGKNADE SKMPAVFFVN WFRRGDDGRF LWPGFGENSR VLKWAIERIE HKADGRSTPI 

       550        560        570        580        590        600 
GIVPTAQDLD LEGLDVDPED VDAALAVKPE EWRQELPLIE EWFEFVGEKL PTGIRDEFDA 


LKHRLAEEA 

« Hide

References

[1]"Complete sequence of chromosome of Mycobacterium sp. KMS."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KMS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000518 Genomic DNA. Translation: ABL89403.1.
RefSeqYP_936193.1. NC_008705.1.

3D structure databases

ProteinModelPortalA1U995.
SMRA1U995. Positions 12-606.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING189918.Mkms_0185.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABL89403; ABL89403; Mkms_0185.
GeneID4615414.
KEGGmkm:Mkms_0185.
PATRIC18099304. VBIMycSp70743_0704.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHOG000191700.
KOK01596.
OMAIDWHGND.
OrthoDBEOG6MPWQS.

Enzyme and pathway databases

BioCycMSP189918:GH4X-188-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00452. PEPCK_GTP.
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERPTHR11561. PTHR11561. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_MYCSK
AccessionPrimary (citable) accession number: A1U995
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways