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A1U995

- PCKG_MYCSK

UniProt

A1U995 - PCKG_MYCSK

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Protein
Phosphoenolpyruvate carboxykinase [GTP]
Gene
pckG, Mkms_0185
Organism
Mycobacterium sp. (strain KMS)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei81 – 811Substrate By similarity
Binding sitei222 – 2221Substrate; via amide nitrogen By similarity
Metal bindingi229 – 2291Manganese By similarity
Binding sitei229 – 2291Substrate By similarity
Metal bindingi249 – 2491Manganese; via tele nitrogen By similarity
Binding sitei271 – 2711Substrate By similarity
Active sitei273 – 2731 By similarity
Metal bindingi296 – 2961Manganese By similarity
Binding sitei389 – 3891GTP By similarity
Binding sitei420 – 4201GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi272 – 2776GTP By similarity
Nucleotide bindingi515 – 5184GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMSP189918:GH4X-188-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEP carboxykinase
Short name:
PEPCK
Gene namesi
Name:pckG
Ordered Locus Names:Mkms_0185
OrganismiMycobacterium sp. (strain KMS)
Taxonomic identifieri189918 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000638: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 609609Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation
PRO_1000060294Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi189918.Mkms_0185.

Structurei

3D structure databases

ProteinModelPortaliA1U995.
SMRiA1U995. Positions 12-606.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni387 – 3893Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiIDWHGND.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1U995-1 [UniParc]FASTAAdd to Basket

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MTSATIPGLD TAPTEHEGLL AWVREVAELT QPDRVVFTDG SEEECARLTE    50
QLCEAGTFQK LNEEKKPNSY LALSDPSDVA RVESRTYICS EREIDAGPTN 100
NWMDPAEMRG IMTDLYRGSM RGRTMYVVPF CMGPLEAEDP KLGVEITDSE 150
YVVVSMRTMT RMGQAALDKM GTDGFFVKAL HSLGAPLEPG EKDVPWPCND 200
TKYITHFPET REIWSYGSGY GGNALLGKKC YSLRIASAMA HDEGWLAEHM 250
LILKLISPEN KAYFIAAAFP SACGKTNLAM LQPTIPGWRA ETVGDDIAWM 300
RFGKDGRLYA VNPEFGFFGV APGTNWSSNP NAMKTIEAGN TVFTNVAKTD 350
DGDVWWEGLE GEPDHLIDWK GNDYILRETE TKAAHPNSRY CTPISQCPTL 400
APEWDDPQGV PISAILFGGR RKTTVPLITQ ARDWQHGVFI GATLGSEQTA 450
AAEGKVGTVR RDPMAMLPFL GYNVGDYFQH WIDIGKNADE SKMPAVFFVN 500
WFRRGDDGRF LWPGFGENSR VLKWAIERIE HKADGRSTPI GIVPTAQDLD 550
LEGLDVDPED VDAALAVKPE EWRQELPLIE EWFEFVGEKL PTGIRDEFDA 600
LKHRLAEEA 609
Length:609
Mass (Da):67,822
Last modified:February 6, 2007 - v1
Checksum:i9F7D9E191F37FA54
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000518 Genomic DNA. Translation: ABL89403.1.
RefSeqiYP_936193.1. NC_008705.1.

Genome annotation databases

EnsemblBacteriaiABL89403; ABL89403; Mkms_0185.
GeneIDi4615414.
KEGGimkm:Mkms_0185.
PATRICi18099304. VBIMycSp70743_0704.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000518 Genomic DNA. Translation: ABL89403.1 .
RefSeqi YP_936193.1. NC_008705.1.

3D structure databases

ProteinModelPortali A1U995.
SMRi A1U995. Positions 12-606.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 189918.Mkms_0185.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABL89403 ; ABL89403 ; Mkms_0185 .
GeneIDi 4615414.
KEGGi mkm:Mkms_0185.
PATRICi 18099304. VBIMycSp70743_0704.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi IDWHGND.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci MSP189918:GH4X-188-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KMS.

Entry informationi

Entry nameiPCKG_MYCSK
AccessioniPrimary (citable) accession number: A1U995
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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