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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Mycobacterium sp. (strain KMS)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107S-adenosyl-L-methionineUniRule annotation1
Binding sitei132S-adenosyl-L-methionineUniRule annotation1
Binding sitei159S-adenosyl-L-methionineUniRule annotation1
Active sitei182By similarity1
Binding sitei182S-adenosyl-L-methionineUniRule annotation1
Binding sitei186SubstrateUniRule annotation1
Binding sitei218SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Mkms_0182
OrganismiMycobacterium sp. (strain KMS)
Taxonomic identifieri189918 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000638 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002881821 – 275tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST275

Structurei

3D structure databases

ProteinModelPortaliA1U992.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni254 – 257Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000073968.
KOiK03439.
OMAiKFEDKAH.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1U992-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRHHGRMHAR ESDVAGGPAR DSAGGPARDS AGGPARDSAG DDSPSAHRHR
60 70 80 90 100
RVTSFRSRRS ALSGGQQDTW ERLWPELGTE ARDDDGRPAA LIDTAAWFGR
110 120 130 140 150
TAPLVLEIGS GTGTSTLAMA QDEPDIDVIA VEVYRRGLAQ LLTGIDRAGV
160 170 180 190 200
RNIRMIRGDG VDVLEYMVAS GSLTGVRVFF PDPWPKARHH KRRLLQPSTV
210 220 230 240 250
ALIADRLRPG GILHAATDHA GYAEQIAAVG DAEPLLRRVD LTDDLPISVR
260 270
RPVTKYERKA LAGPDVAELL WEKVP
Length:275
Mass (Da):29,801
Last modified:February 6, 2007 - v1
Checksum:iE9B0DF08386B98A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA. Translation: ABL89400.1.
RefSeqiWP_011557600.1. NC_008705.1.

Genome annotation databases

EnsemblBacteriaiABL89400; ABL89400; Mkms_0182.
KEGGimkm:Mkms_0182.
PATRICi18099298. VBIMycSp70743_0701.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000518 Genomic DNA. Translation: ABL89400.1.
RefSeqiWP_011557600.1. NC_008705.1.

3D structure databases

ProteinModelPortaliA1U992.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABL89400; ABL89400; Mkms_0182.
KEGGimkm:Mkms_0182.
PATRICi18099298. VBIMycSp70743_0701.

Phylogenomic databases

HOGENOMiHOG000073968.
KOiK03439.
OMAiKFEDKAH.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_MYCSK
AccessioniPrimary (citable) accession number: A1U992
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 6, 2007
Last modified: November 2, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.