A1U965 (A1U965_MYCSK) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase HAMAP MF_00012
Short name=DAD HAMAP MF_00012
EC=4.2.1.9 HAMAP MF_00012

Gene names

Name:	ilvD HAMAP MF_00012
Ordered Locus Names:	Mkms_0155

Organism

Mycobacterium sp. (strain KMS) [Complete proteome] [HAMAP]

Taxonomic identifier

189918 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family. HAMAP MF_00012

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00012
Ligand	4Fe-4S HAMAP MF_00012 Iron Iron-sulfur Metal-binding
Molecular function	Lyase HAMAP MF_00012 EMBL ABL89373.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	142	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012
	Metal binding	219	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012

Sequences

	Sequence		Length	Mass (Da)
	A1U965 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: B639D19528025E5E	FASTA	580	60,522
	10 20 30 40 50 60 MPSGSSGTPA DALRASDSSP DIKPRSRDVT DGLEKTAARG MLRAVGMGDD DWVKPQIGVG 70 80 90 100 110 120 SSWNEITPCN MSLQRLAQSV KGGVHSAGGF PLEFGTISVS DGISMGHEGM HFSLVSREVI 130 140 150 160 170 180 ADSVETVVQA ERLDGTVLLA GCDKSIPGML MAAARLDLAS VFLYNGSIMP GVAKLTDGSE 190 200 210 220 230 240 REVTIIDAFE AVGACARGLM SREDVDIIER AICPGEGACG GMYTANTMAS AAEALGMSLP 250 260 270 280 290 300 GSASPVAIDK RRDEYARKSG EAVVEMLRRG ITARDILTKE AFENAIAVVM AFGGSTNAVL 310 320 330 340 350 360 HLLAIAWEAN VKLTLEDFTR VGQKVPHLAD VKPFGRHVMK HVDEIGGVPV VMKALLDAGL 370 380 390 400 410 420 LHGDCLTITG ETMAENLAHI EPPDPDGKVL RAMNNPIHPT GGITILHGSL APEGAVVKSA 430 440 450 460 470 480 GFDSDVFEGT ARVFERERAA LDALEDGTIT HGDVVVIRYE GPKGGPGMRE MLAITGAIKG 490 500 510 520 530 540 AGLGKDVLLM TDGRFSGGTT GLCVGHIAPE AVDGGPIAFV RDGDRIRLDV AKGTLDLLVD 550 560 570 580 EAEFESRKAG FEPLPPVYTT GVLAKYTKLV GSAAVGAVCG

References

[1]

"Complete sequence of chromosome of Mycobacterium sp. KMS." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KMS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000518 Genomic DNA. Translation: ABL89373.1.
RefSeq	YP_936163.1. NC_008705.1.
3D structure databases
ProteinModelPortal	A1U965.
ModBase	Search...
Protein-protein interaction databases
STRING	A1U965.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000056460; EBMYCP00000054684; EBMYCG00000056455.
GeneID	4615561.
GenomeReviews	Gene locus Mkms_0155 in contig CP000518_GR.
KEGG	mkm:Mkms_0155.
PATRIC	18099242. VBIMycSp70743_0673.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
GeneTree	EBGT00050000016366.
HOGENOM	HBG671001.
OMA	NMPGAMI.
ProtClustDB	PRK00911.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A1U965_MYCSK

Accession

Primary (citable) accession number: A1U965

Entry history

Integrated into UniProtKB/TrEMBL:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)