A1U8S0 (DNAA_MYCSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	Mkms_0009

Organism

Mycobacterium sp. (strain KMS) [Complete proteome] [HAMAP]

Taxonomic identifier

189918 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	Chromosomal replication initiator protein DnaA HAMAP MF_00377		PRO_1000048672
Regions
	Nucleotide binding	196 – 203	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A1U8S0 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: CA67241F800798EB	FASTA	495	55,645
	10 20 30 40 50 60 MTADPDPPFV AVWNTVVAEL NGDPAATGPR NGDGALPTLT PQQRAWLKLV KPLVITEGFA 70 80 90 100 110 120 LLSVPTPFVQ NEIERHLREP IITALSRHLG QRVELGVRIA TPSPEDDDPP PSPVIADIDE 130 140 150 160 170 180 VDEDTEARVS AEETWPRYFS RPPETPAAED PNAVSLNRRY TFDTFVIGAS NRFAHAATLA 190 200 210 220 230 240 IAEAPARAYN PLFIWGESGL GKTHLLHAAG NYAQRLFPGM RVKYVSTEEF TNDFINSLRD 250 260 270 280 290 300 DRKASFKRSY RDIDVLLVDD IQFIEGKEGI QEEFFHTFNT LHNANKQIVI SSDRPPKQLA 310 320 330 340 350 360 TLEDRLRTRF EWGLITDVQP PELETRIAIL RKKAQMDRLD VPDDVLELIA SSIERNIREL 370 380 390 400 410 420 EGALIRVTAF ASLNKTPIDK SLAEIVLRDL ISDASTMQIS TAAIMAATAE YFETTIEELR 430 440 450 460 470 480 GPGKTRALAQ SRQIAMYLCR ELTDLSLPKI GQAFGRDHTT VMYAEKKIRG EMAERREVFD 490 HVKELTTRIR QRAKR

References

[1]

"Complete sequence of chromosome of Mycobacterium sp. KMS." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KMS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000518 Genomic DNA. Translation: ABL89228.1.
RefSeq	YP_936018.1. NC_008705.1.
3D structure databases
ProteinModelPortal	A1U8S0.
ModBase	Search...
Protein-protein interaction databases
STRING	A1U8S0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000053382; EBMYCP00000051606; EBMYCG00000053377.
GeneID	4615573.
GenomeReviews	Gene locus Mkms_0009 in contig CP000518_GR.
KEGG	mkm:Mkms_0009.
PATRIC	18098942. VBIMycSp70743_0526.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0593.
GeneTree	EBGT00050000017320.
HOGENOM	HBG695666.
OMA	DEVAFLI.
ProtClustDB	PRK00149.
Enzyme and pathway databases
BioCyc	MSP189918:MKMS_0009-MONOMER.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_MYCSK

Accession

Primary (citable) accession number: A1U8S0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program