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Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

Miscellaneous

The active site is located at the dimer interface.UniRule annotation

Catalytic activityi

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

Cofactori

Zn2+UniRule annotation, Mg2+UniRule annotation, Co2+UniRule annotationNote: Binds 1 divalent metal cation per subunit. Can use ions such as Zn2+, Mg2+ or Co2+.UniRule annotation

Pathwayi: pyridoxine 5'-phosphate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Erythronate-4-phosphate dehydrogenase (pdxB)
  3. Phosphoserine aminotransferase (serC)
  4. 4-hydroxythreonine-4-phosphate dehydrogenase (pdxA)
  5. Pyridoxine 5'-phosphate synthase (pdxJ)
This subpathway is part of the pathway pyridoxine 5'-phosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyridoxine 5'-phosphate from D-erythrose 4-phosphate, the pathway pyridoxine 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei135SubstrateUniRule annotation1
Binding sitei136SubstrateUniRule annotation1
Metal bindingi165Divalent metal cation; shared with dimeric partnerUniRule annotation1
Metal bindingi210Divalent metal cation; shared with dimeric partnerUniRule annotation1
Metal bindingi265Divalent metal cation; shared with dimeric partnerUniRule annotation1
Binding sitei273SubstrateUniRule annotation1
Binding sitei282SubstrateUniRule annotation1
Binding sitei291SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPyridoxine biosynthesis
LigandCobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

Enzyme and pathway databases

UniPathwayiUPA00244; UER00312.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.262UniRule annotation)
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenaseUniRule annotation
Gene namesi
Name:pdxAUniRule annotation
Ordered Locus Names:Maqu_3508
OrganismiMarinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
Taxonomic identifieri351348 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeMarinobacter
Proteomesi
  • UP000000998 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000515071 – 3364-hydroxythreonine-4-phosphate dehydrogenaseAdd BLAST336

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi351348.Maqu_3508.

Structurei

3D structure databases

ProteinModelPortaliA1U6F9.
SMRiA1U6F9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PdxA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CEZ. Bacteria.
COG1995. LUCA.
HOGENOMiHOG000221592.
KOiK00097.
OMAiAPVHKGV.
OrthoDBiPOG091H03XD.

Family and domain databases

HAMAPiMF_00536. PdxA. 1 hit.
InterProiView protein in InterPro
IPR005255. PdxA.
PANTHERiPTHR30004. PTHR30004. 1 hit.
PfamiView protein in Pfam
PF04166. PdxA. 1 hit.
TIGRFAMsiTIGR00557. pdxA. 1 hit.

Sequencei

Sequence statusi: Complete.

A1U6F9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPVILALT AGEPAGIGPE LCLQLATGSR DAGLVVIASG ELLRDRASQL
60 70 80 90 100
GLQIELHDWQ PGVAAATRAG HLSVRHVEGC GSTVAGRLDK ANSQYVLDTL
110 120 130 140 150
TIAARGCLNG DFDGMVTAPV HKGVINEAGI VFSGHTEFLQ ELCGVERVVM
160 170 180 190 200
MLATEELRVA LVTTHLPLKD VSAAITPDRL TQVTRILNAD LKAFFGIDQP
210 220 230 240 250
RILVAGLNPH AGEGGHLGRE EIEVIEPTLE QLRAEGIQLT GPLPADTLFT
260 270 280 290 300
PHWLDNADAV LAMYHDQGLP VLKFQGFGRA VNITLGLPIV RTSVDHGTAL
310 320 330
DLAGTGKADA GSLHTAIRVG EQMAQCRKAT GAGELS
Length:336
Mass (Da):35,346
Last modified:February 6, 2007 - v1
Checksum:iEEBB9ED10E708F1F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000514 Genomic DNA. Translation: ABM20578.1.
RefSeqiWP_011786919.1. NC_008740.1.

Genome annotation databases

EnsemblBacteriaiABM20578; ABM20578; Maqu_3508.
KEGGimaq:Maqu_3508.

Similar proteinsi

Entry informationi

Entry nameiPDXA_MARHV
AccessioniPrimary (citable) accession number: A1U6F9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: September 27, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families