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A1U4B2 (BIOB_MARAV) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein attributes

Sequence length352 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 352352Biotin synthase HAMAP-Rule MF_01694
PRO_0000381454

Sites

Metal binding561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1001Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1311Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1911Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2631Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A1U4B2 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 7666DFC600F5B2BD

FASTA35239,889
        10         20         30         40         50         60 
MTATATRHDW TLQEARELFN LPFNDLLFRA QSIHREHFDP NEVQVSTLLS IKTGACPEDC 

        70         80         90        100        110        120 
KYCPQSGHYN TGLEKEKLLE IEKVVAEARV AREKGASRFC MGAAWRSPSK KDMPYVLDMV 

       130        140        150        160        170        180 
RQVKSLGLET CMTLGMLKEE EAKELADAGL DYYNHNLDTS EKYYNHIITT RTYQDRLDTL 

       190        200        210        220        230        240 
DNVRKAGMKV CCGGIMGMGE DEDDRVGLLV QLANLPQHPE SVPVNMLVKV KGTPLEDVED 

       250        260        270        280        290        300 
LDPFDFIRII AVARIMMPAS HVRLSAGREQ MNEQMQALCF MAGANSIFYG EKLLTTSNPE 

       310        320        330        340        350 
ADADMQLFRK LGIRPEQREQ CATEEQEEEA IAEAVEYEAT RHMFYDATRE SA 

« Hide

References

[1]"Complete sequence of chromosome 1 of Marinobacter aquaeolei VT8."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Edwards K., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700491 / DSM 11845 / VT8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000514 Genomic DNA. Translation: ABM19831.1.
RefSeqYP_960018.1. NC_008740.1.

3D structure databases

ProteinModelPortalA1U4B2.
SMRA1U4B2. Positions 7-317.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351348.Maqu_2756.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM19831; ABM19831; Maqu_2756.
GeneID4656018.
KEGGmaq:Maqu_2756.
PATRIC22460858. VBIMarAqu65105_3171.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2311234.

Enzyme and pathway databases

BioCycMHYD351348:GHYZ-2815-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_MARAV
AccessionPrimary (citable) accession number: A1U4B2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 6, 2007
Last modified: February 19, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways