Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A1U4B2

- BIOB_MARHV

UniProt

A1U4B2 - BIOB_MARHV

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Biotin synthase

Gene

bioB

Organism
Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi100 – 1001Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi131 – 1311Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi191 – 1911Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi263 – 2631Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMHYD351348:GHYZ-2815-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Maqu_2756
OrganismiMarinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
Taxonomic identifieri351348 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeMarinobacter
ProteomesiUP000000998: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 352352Biotin synthasePRO_0000381454Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi351348.Maqu_2756.

Structurei

3D structure databases

ProteinModelPortaliA1U4B2.
SMRiA1U4B2. Positions 7-317.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A1U4B2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTATATRHDW TLQEARELFN LPFNDLLFRA QSIHREHFDP NEVQVSTLLS
60 70 80 90 100
IKTGACPEDC KYCPQSGHYN TGLEKEKLLE IEKVVAEARV AREKGASRFC
110 120 130 140 150
MGAAWRSPSK KDMPYVLDMV RQVKSLGLET CMTLGMLKEE EAKELADAGL
160 170 180 190 200
DYYNHNLDTS EKYYNHIITT RTYQDRLDTL DNVRKAGMKV CCGGIMGMGE
210 220 230 240 250
DEDDRVGLLV QLANLPQHPE SVPVNMLVKV KGTPLEDVED LDPFDFIRII
260 270 280 290 300
AVARIMMPAS HVRLSAGREQ MNEQMQALCF MAGANSIFYG EKLLTTSNPE
310 320 330 340 350
ADADMQLFRK LGIRPEQREQ CATEEQEEEA IAEAVEYEAT RHMFYDATRE

SA
Length:352
Mass (Da):39,889
Last modified:February 6, 2007 - v1
Checksum:i7666DFC600F5B2BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000514 Genomic DNA. Translation: ABM19831.1.
RefSeqiYP_960018.1. NC_008740.1.

Genome annotation databases

EnsemblBacteriaiABM19831; ABM19831; Maqu_2756.
GeneIDi4656018.
KEGGimaq:Maqu_2756.
PATRICi22460858. VBIMarAqu65105_3171.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000514 Genomic DNA. Translation: ABM19831.1 .
RefSeqi YP_960018.1. NC_008740.1.

3D structure databases

ProteinModelPortali A1U4B2.
SMRi A1U4B2. Positions 7-317.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 351348.Maqu_2756.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABM19831 ; ABM19831 ; Maqu_2756 .
GeneIDi 4656018.
KEGGi maq:Maqu_2756.
PATRICi 22460858. VBIMarAqu65105_3171.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MHYD351348:GHYZ-2815-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Marinobacter aquaeolei VT8."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.
    , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Edwards K., Richardson P.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700491 / DSM 11845 / VT8.

Entry informationi

Entry nameiBIOB_MARHV
AccessioniPrimary (citable) accession number: A1U4B2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 6, 2007
Last modified: October 29, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3