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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei623 – 6231ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMHYD351348:GHYZ-2803-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Maqu_2744
OrganismiMarinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
Taxonomic identifieri351348 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeMarinobacter
ProteomesiUP000000998: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 861861Leucine--tRNA ligasePRO_0000334770Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi351348.Maqu_2744.

Structurei

3D structure databases

ProteinModelPortaliA1U4A0.
SMRiA1U4A0. Positions 229-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi620 – 6245"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1U4A0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDEQYNPREV EQTARTFWET NETFKVKEEP GKPKYYCLSM FPYPSGKLHM
60 70 80 90 100
GHVRNYTIGD VISRYQRMQG KNVMQPMGWD AFGLPAENAA IANKSAPAKW
110 120 130 140 150
TRSNIDYMKN QLKQLGFGYD WSRELATCDP EYYRWEQWFF ARLYEKGLVY
160 170 180 190 200
KKMSTVNWDP VDQTVLANEQ VVDGRGWRSG ALVEQKKIPQ WFIRITDYAE
210 220 230 240 250
ELLNDLEDME GWPEQVKTMQ RNWIGKSVGT ELTFPLKEQE GGLTVYTTRP
260 270 280 290 300
DTLMGVSYMA VAAEHPLAKA AAERHRDVAE FVDQCRNSKV AEAELATMEK
310 320 330 340 350
KGIDTGFKAI HPLTQEEIPV WVANFVLMDY GTGALMAVPG HDERDHEFAV
360 370 380 390 400
KYKLPIKQVI APNDSRDIDI QEQAFTEKGV LVSSGKYSGL TSEEAFEEIA
410 420 430 440 450
DYLEAQGIGK RTVNYRLRDW GVSRQRYWGA PIPMMTLEDG TEMPVPDDQL
460 470 480 490 500
PVRLPEDVEM DGVQSPIKAD PEWCKTEYNG QPATLETDTF DTFMESSWYY
510 520 530 540 550
ARFCSPNYDK GMLDPAAANY WLPVDQYIGG IEHAILHLLY ARFFHKLLRD
560 570 580 590 600
VGLVNSSEPF KRLLTQGMVL AETYYRNEAN GGKTWYNPAD VTVERDGKGQ
610 620 630 640 650
VVRAVLKSDG EPVEIGGVTK MSKSKNNGID PQAIIDQHGA DTVRLFMMFA
660 670 680 690 700
APPEQSLEWS DSGVEGAHRF LKRLWRMVNE HTAGGDAPAV DPANLNGAQK
710 720 730 740 750
DLRRKTHETI AKVSDDISRR LTFNTAIAAV MELLNEVGRL SDDEPQSRAV
760 770 780 790 800
RQEALDTAVL VLSPIVPHIC HNLWQALGHS DAVVDASWPV ADESAMVRSE
810 820 830 840 850
IEVVLQVNGK VRAKENVPAD IGKADLEKLA LENENVMRFT DGKTVRKVIV
860
VPGKLVNVVA N
Length:861
Mass (Da):97,230
Last modified:May 20, 2008 - v2
Checksum:i0C308BF782455E9A
GO

Sequence cautioni

The sequence ABM19819.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000514 Genomic DNA. Translation: ABM19819.1. Different initiation.
RefSeqiYP_960006.1. NC_008740.1.

Genome annotation databases

EnsemblBacteriaiABM19819; ABM19819; Maqu_2744.
GeneIDi4654614.
KEGGimaq:Maqu_2744.
PATRICi22460834. VBIMarAqu65105_3159.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000514 Genomic DNA. Translation: ABM19819.1. Different initiation.
RefSeqiYP_960006.1. NC_008740.1.

3D structure databases

ProteinModelPortaliA1U4A0.
SMRiA1U4A0. Positions 229-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351348.Maqu_2744.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM19819; ABM19819; Maqu_2744.
GeneIDi4654614.
KEGGimaq:Maqu_2744.
PATRICi22460834. VBIMarAqu65105_3159.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciMHYD351348:GHYZ-2803-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Marinobacter aquaeolei VT8."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.
    , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Edwards K., Richardson P.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700491 / DSM 11845 / VT8.

Entry informationi

Entry nameiSYL_MARHV
AccessioniPrimary (citable) accession number: A1U4A0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: January 7, 2015
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.