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A1U4A0 (SYL_MARAV) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Maqu_2744
OrganismMarinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) (Marinobacter hydrocarbonoclasticus (strain DSM 11845)) [Complete proteome] [HAMAP]
Taxonomic identifier351348 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeMarinobacter

Protein attributes

Sequence length861 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence ABM19819.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 861861Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334770

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif620 – 6245"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6231ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1U4A0 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: 0C308BF782455E9A

FASTA86197,230
        10         20         30         40         50         60 
MDEQYNPREV EQTARTFWET NETFKVKEEP GKPKYYCLSM FPYPSGKLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMQG KNVMQPMGWD AFGLPAENAA IANKSAPAKW TRSNIDYMKN QLKQLGFGYD 

       130        140        150        160        170        180 
WSRELATCDP EYYRWEQWFF ARLYEKGLVY KKMSTVNWDP VDQTVLANEQ VVDGRGWRSG 

       190        200        210        220        230        240 
ALVEQKKIPQ WFIRITDYAE ELLNDLEDME GWPEQVKTMQ RNWIGKSVGT ELTFPLKEQE 

       250        260        270        280        290        300 
GGLTVYTTRP DTLMGVSYMA VAAEHPLAKA AAERHRDVAE FVDQCRNSKV AEAELATMEK 

       310        320        330        340        350        360 
KGIDTGFKAI HPLTQEEIPV WVANFVLMDY GTGALMAVPG HDERDHEFAV KYKLPIKQVI 

       370        380        390        400        410        420 
APNDSRDIDI QEQAFTEKGV LVSSGKYSGL TSEEAFEEIA DYLEAQGIGK RTVNYRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMMTLEDG TEMPVPDDQL PVRLPEDVEM DGVQSPIKAD PEWCKTEYNG 

       490        500        510        520        530        540 
QPATLETDTF DTFMESSWYY ARFCSPNYDK GMLDPAAANY WLPVDQYIGG IEHAILHLLY 

       550        560        570        580        590        600 
ARFFHKLLRD VGLVNSSEPF KRLLTQGMVL AETYYRNEAN GGKTWYNPAD VTVERDGKGQ 

       610        620        630        640        650        660 
VVRAVLKSDG EPVEIGGVTK MSKSKNNGID PQAIIDQHGA DTVRLFMMFA APPEQSLEWS 

       670        680        690        700        710        720 
DSGVEGAHRF LKRLWRMVNE HTAGGDAPAV DPANLNGAQK DLRRKTHETI AKVSDDISRR 

       730        740        750        760        770        780 
LTFNTAIAAV MELLNEVGRL SDDEPQSRAV RQEALDTAVL VLSPIVPHIC HNLWQALGHS 

       790        800        810        820        830        840 
DAVVDASWPV ADESAMVRSE IEVVLQVNGK VRAKENVPAD IGKADLEKLA LENENVMRFT 

       850        860 
DGKTVRKVIV VPGKLVNVVA N 

« Hide

References

[1]"Complete sequence of chromosome 1 of Marinobacter aquaeolei VT8."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Edwards K., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700491 / DSM 11845 / VT8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000514 Genomic DNA. Translation: ABM19819.1. Different initiation.
RefSeqYP_960006.1. NC_008740.1.

3D structure databases

ProteinModelPortalA1U4A0.
SMRA1U4A0. Positions 229-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351348.Maqu_2744.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM19819; ABM19819; Maqu_2744.
GeneID4654614.
KEGGmaq:Maqu_2744.
PATRIC22460834. VBIMarAqu65105_3159.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycMHYD351348:GHYZ-2803-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_MARAV
AccessionPrimary (citable) accession number: A1U4A0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries