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A1U3F6 (DDL_MARAV) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Maqu_2450
OrganismMarinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) (Marinobacter hydrocarbonoclasticus (strain DSM 11845)) [Complete proteome] [HAMAP]
Taxonomic identifier351348 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeMarinobacter

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 325325D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341129

Regions

Domain121 – 316196ATP-grasp
Nucleotide binding147 – 20256ATP By similarity

Sites

Metal binding2701Magnesium or manganese 1 By similarity
Metal binding2831Magnesium or manganese 1 By similarity
Metal binding2831Magnesium or manganese 2 By similarity
Metal binding2851Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A1U3F6 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 9620927A2177ED76

FASTA32534,906
        10         20         30         40         50         60 
MSDLTHNDIQ PYQAHPELVR ALGRVAVFMG GDSAEREVSL KSGKAVLAAL QSAGIDAVGR 

        70         80         90        100        110        120 
DIQGCLLRTV DEPDFDRVFI ALHGRGGEDG TLQAILGQAG IPYTGSEVLA SALAMDKLRT 

       130        140        150        160        170        180 
KYVFEGCGLP TPAFRAMTSV GEAEGIVAAL GTPLSVKPAH EGSSIGIRKV NSAAELAEAY 

       190        200        210        220        230        240 
EAAARLDDLV LVEQWIEGPE FTVSLLQDKA LPAIGLSTDH AFYDYDAKYL ADDTRYRIPC 

       250        260        270        280        290        300 
GLAPDDELRL QHLALDAFRV LGCRTWGRVD IMQDRAGEFW LLEVNTVPGM TDHSLVPMAA 

       310        320 
KAAGISFEEL VVRILRDTLA EGIDA 

« Hide

References

[1]"Complete sequence of chromosome 1 of Marinobacter aquaeolei VT8."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Edwards K., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700491 / DSM 11845 / VT8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000514 Genomic DNA. Translation: ABM19525.1.
RefSeqYP_959712.1. NC_008740.1.

3D structure databases

ProteinModelPortalA1U3F6.
SMRA1U3F6. Positions 23-316.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351348.Maqu_2450.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM19525; ABM19525; Maqu_2450.
GeneID4656929.
KEGGmaq:Maqu_2450.
PATRIC22460242. VBIMarAqu65105_2870.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycMHYD351348:GHYZ-2501-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_MARAV
AccessionPrimary (citable) accession number: A1U3F6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: February 6, 2007
Last modified: February 19, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways