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Protein

Thymidylate synthase

Gene

thyA

Organism
Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21dUMPUniRule annotation1
Binding sitei515,10-methylenetetrahydrofolateUniRule annotation1
Active sitei159NucleophileUniRule annotation1
Binding sitei1825,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei190dUMPUniRule annotation1
Binding sitei2785,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi126 – 127dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi179 – 182dUMPUniRule annotation4
Nucleotide bindingi220 – 222dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:Maqu_2432
OrganismiMarinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
Taxonomic identifieri351348 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeMarinobacter
Proteomesi
  • UP000000998 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000006231 – 279Thymidylate synthaseAdd BLAST279

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi351348.Maqu_2432.

Structurei

3D structure databases

ProteinModelPortaliA1U3D8.
SMRiA1U3D8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V. Bacteria.
COG0207. LUCA.
HOGENOMiHOG000257899.
KOiK00560.
OMAiKQYLDLC.
OrthoDBiPOG091H02L6.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiView protein in InterPro
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
PfamiView protein in Pfam
PF00303. Thymidylat_synt. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.

Sequencei

Sequence statusi: Complete.

A1U3D8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAYLDLMQD VVDNGFDKGD RTGVGTRSVF GRQMRFNLQD GFPLVTTKKV
60 70 80 90 100
HLRSIIYELL WFLKGSTDNT WLAERKVSIW NEWALENGDL GPIYGKQWRS
110 120 130 140 150
WQCHDGRVID QISEVIEQIR TKPNSRRLIV SAWNPAELPD ESISPQDNVR
160 170 180 190 200
EGRMALAPCH CLFQFYVADG KLSCQLYQRS ADLFLGVPFN IASYALLTHM
210 220 230 240 250
IAQQCDLDVG EFVHTFGDCH LYRNHLTDDI VFEQLRREPR ALPKLVIKRK
260 270
PESIFGYELE DFEFEGYDPS PVIKAPIAI
Length:279
Mass (Da):32,173
Last modified:February 6, 2007 - v1
Checksum:i3C82F31D525BCECB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000514 Genomic DNA. Translation: ABM19507.1.
RefSeqiWP_011785891.1. NC_008740.1.

Genome annotation databases

EnsemblBacteriaiABM19507; ABM19507; Maqu_2432.
KEGGimaq:Maqu_2432.

Similar proteinsi

Entry informationi

Entry nameiTYSY_MARHV
AccessioniPrimary (citable) accession number: A1U3D8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: August 30, 2017
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families