A1U326 (GLPK_MARAV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Maqu_2319

Organism

Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) (Marinobacter hydrocarbonoclasticus (strain DSM 11845)) [Complete proteome] [HAMAP]

Taxonomic identifier

351348 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Marinobacter

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Glycerol kinase HAMAP MF_00186		PRO_1000020744
Regions
	Nucleotide binding	408 – 412	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	265	1	ATP By similarity
	Binding site	308	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1U326 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: D31615971DEC61A5	FASTA	493	53,960
	10 20 30 40 50 60 MSRYLLAIDQ GTTSSRAIVF DDKGAQVAVA QQEFHQYFPK DGWVEHDAME IWDSTLAVCR 70 80 90 100 110 120 AALNNGKIKA SDLAGIGITN QRETTVIWDR ATGQPIHHAI VWQDRRTASW CTKLRQDGHE 130 140 150 160 170 180 SLVVDRTGLL IDPYFSATKI AWILDNVDGA RQRAEAGELA FGTIDTWLLW NLTGGKHHRT 190 200 210 220 230 240 DATNASRTAL FNIHTQQWDP QLLKLFRVPA ALLPEVFDSA AEYGLTEPEC LGASVMIAGI 250 260 270 280 290 300 AGDQHAALIG QACFEPGMAK STYGTGCFLM LNTGQQALRS ENRLLTTVAY RLNGKPCYAI 310 320 330 340 350 360 EGSIFVAGAA MQWLRDGLRL ISHARESAEH AATVGVNNPV YLVPAFTGLG APHWDPNARG 370 380 390 400 410 420 AILGLTRDTG IAEIVTAGLQ SVCYQTKDLV RAIRNDGARL ESLRVDGGMA VNDWVMQFLS 430 440 450 460 470 480 DILNVPVDRP SVTETTALGA AYLAGLQTGV FDSLEQITSL WECEHQFRPD MAPGLRESLY 490 AGWLDAVERV CNH

References

[1]

"Complete sequence of chromosome 1 of Marinobacter aquaeolei VT8." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Edwards K., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700491 / DSM 11845 / VT8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000514 Genomic DNA. Translation: ABM19395.1.
RefSeq	YP_959582.1. NC_008740.1.
3D structure databases
ProteinModelPortal	A1U326.
SMR	A1U326. Positions 4-491.
ModBase	Search...
Protein-protein interaction databases
STRING	A1U326.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4656406.
GenomeReviews	Gene locus Maqu_2319 in contig CP000514_GR.
KEGG	maq:Maqu_2319.
PATRIC	22459964. VBIMarAqu65105_2734.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0554.
HOGENOM	HBG511469.
OMA	MEMLPEV.
PhylomeDB	A1U326.
Enzyme and pathway databases
BioCyc	MAQU351348:MAQU_2319-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_MARAV

Accession

Primary (citable) accession number: A1U326

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program