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Protein

Malate synthase G

Gene

glcB

Organism
Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei276 – 2761Acetyl-CoAUniRule annotation
Binding sitei313 – 3131Acetyl-CoAUniRule annotation
Active sitei340 – 3401Proton acceptorUniRule annotation
Binding sitei340 – 3401GlyoxylateUniRule annotation
Metal bindingi432 – 4321MagnesiumUniRule annotation
Binding sitei432 – 4321GlyoxylateUniRule annotation
Metal bindingi460 – 4601MagnesiumUniRule annotation
Binding sitei541 – 5411Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei632 – 6321Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMHYD351348:GHYZ-1883-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Maqu_1843
OrganismiMarinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
Taxonomic identifieri351348 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeMarinobacter
ProteomesiUP000000998 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 726726Malate synthase GPRO_1000130890Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei618 – 6181Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi351348.Maqu_1843.

Structurei

3D structure databases

ProteinModelPortaliA1U1Q6.
SMRiA1U1Q6. Positions 2-726.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
Regioni457 – 4604Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

A1U1Q6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERVQVGGI QIAKNLYDFV NNEAIPGTGI EADKFWAEFD KIVNELAPRN
60 70 80 90 100
RELLLKRDEI QEKMDTWNRE HKGQKLDMAE YKAFLKDIGY LVDEPADFKI
110 120 130 140 150
STSNVDPEIA TMAGPQLVVP VMNARFALNA ANARWGSLYD ALYGTDAISE
160 170 180 190 200
ENGAEKGRGY NPVRGAKVIE WARNLLDGSA PLASGSHKDA AKYYIDGGKL
210 220 230 240 250
AVKLQNGDVT GLKDESGFVG YTGAADAPTG VLLVKNGMHF EIQIDASHPI
260 270 280 290 300
GKDDGAHVKD VLMESALTTI MDCEDSVAAV DADDKVVAYK NWLGLMKGDL
310 320 330 340 350
EESFEKGGQM MTRRMNGDRT YTGADGSELT LKGRSLLFVR NVGHLMTNPA
360 370 380 390 400
ILLKDGSEIP EGLMDGLVTS LIAIHDLKGD GKFQNSTKGS VYIVKPKQHG
410 420 430 440 450
PEEVAFTNEF FGRVEDALGL PRFTLKVGIM DEERRTTVNL KACIHAAKER
460 470 480 490 500
TVFINTGFLD RTGDEIHTSM ELGPFIRKGP MKQAAWINAY EQWNVDIGLE
510 520 530 540 550
AGLSGVAQIG KGMWAMPDLM AGMLEAKIGH PKAGANTAWV PSPTAATLHA
560 570 580 590 600
THYHQVNVFD VQKELANRQR ASLDDILTVP VMEDPSSLSA EDIQQELDNN
610 620 630 640 650
AQGILGYVVR WIDQGVGCSK VPDINDVGLM EDRATLRISS QLLTNWLYHG
660 670 680 690 700
ICSEEQIMET MKRMAAVVDK QNAGDAAYRN MAPNFDDSIA FQAAVDLVLK
710 720
GREQPAGYTE PLLHAYRQKA KAKYGQ
Length:726
Mass (Da):79,629
Last modified:February 6, 2007 - v1
Checksum:iC283C1C0799B0F19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000514 Genomic DNA. Translation: ABM18925.1.
RefSeqiWP_011785321.1. NC_008740.1.
YP_959112.1. NC_008740.1.

Genome annotation databases

EnsemblBacteriaiABM18925; ABM18925; Maqu_1843.
KEGGimaq:Maqu_1843.
PATRICi22458986. VBIMarAqu65105_2253.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000514 Genomic DNA. Translation: ABM18925.1.
RefSeqiWP_011785321.1. NC_008740.1.
YP_959112.1. NC_008740.1.

3D structure databases

ProteinModelPortaliA1U1Q6.
SMRiA1U1Q6. Positions 2-726.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351348.Maqu_1843.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM18925; ABM18925; Maqu_1843.
KEGGimaq:Maqu_1843.
PATRICi22458986. VBIMarAqu65105_2253.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciMHYD351348:GHYZ-1883-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Marinobacter aquaeolei VT8."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.
    , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Edwards K., Richardson P.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700491 / DSM 11845 / VT8.

Entry informationi

Entry nameiMASZ_MARHV
AccessioniPrimary (citable) accession number: A1U1Q6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 6, 2007
Last modified: May 27, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.