A1TZH9 (KATG_MARAV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Maqu_1056

Organism

Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) (Marinobacter hydrocarbonoclasticus (strain DSM 11845)) [Complete proteome] [HAMAP]

Taxonomic identifier

351348 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Marinobacter

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 723	723	Catalase-peroxidase HAMAP MF_01961		PRO_0000354828
Sites
	Active site	97	1	Proton acceptor By similarity
	Metal binding	265	1	Iron (heme axial ligand) By similarity
	Site	93	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	96 ↔ 224		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-250) By similarity
	Cross-link	224 ↔ 250		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TZH9 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 3DB66A4DFF46D9B4	FASTA	723	80,764
	10 20 30 40 50 60 MSETNSGGKC PVMHGANSTE NRDVMNWWPE ALNLDILHQH DHKTNPMGED FDYREEVKKL 70 80 90 100 110 120 DFEAVKKDLH ALMTDSQDWW PADWGHYGGL MIRMSWHAAG TYRIQDGRGG GGTGNQRFAP 130 140 150 160 170 180 LNSWPDNVSL DKARRLLWPI KKKYGNKLSW ADLIILAGTV AYESMGLPSY GFSFGREDIW 190 200 210 220 230 240 HPEKDIYWGN EKEWLAPSDE RYGDVEKPDT MENPLAAVQM GLIYVNPEGV NSQPDPIKTG 250 260 270 280 290 300 EQVRVTFARM AMDDEETAAL TAGGHTVGKC HGNGDAGALG PEPEAADVEE QGFGWSNPNM 310 320 330 340 350 360 KGKAANAVTS GIEGAWTTHP TKFDMGYFDL LFGYEWELKK SPAGAWQWEP INMKEEDKPL 370 380 390 400 410 420 DASDPSKRHN PIMTDADMAM KMDPKYRAIC EKFMADPEYF KDCFARAWFK LTHRDLGPKA 430 440 450 460 470 480 RYIGPEAPAE DLIWQDPVPA GSTDYCEEVV KEKVADSGLS ISEMVSTAWD SARTFRGSDM 490 500 510 520 530 540 RGGANGARIR LAPQKDWEGN EPARLSKVLK VYEQISAETG ASVADVIVLA GSVGIEKAAK 550 560 570 580 590 600 AAGYEVRVPF LKGRGDATDE MTDAPSFEYL EPVADGFRNW QKKDYIVKPE ELLLDRAQLM 610 620 630 640 650 660 GLTAPEMTVL IGGMRVLGTN HGGTKYGVFT DREGQLTNDF FVNLTDMAYS WKPVGNGTYE 670 680 690 700 710 720 IRDRKTDQTK WTASRVDLVF GSNSILRSYA EVYAQDDNGE KFVKDFIAAW TKVMNNDRFD VQA

References

[1]

"Complete sequence of chromosome 1 of Marinobacter aquaeolei VT8." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Edwards K., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700491 / DSM 11845 / VT8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000514 Genomic DNA. Translation: ABM18148.1.
RefSeq	YP_958335.1. NC_008740.1.
3D structure databases
ProteinModelPortal	A1TZH9.
SMR	A1TZH9. Positions 27-721.
ModBase	Search...
Protein-protein interaction databases
STRING	A1TZH9.
Protein family/group databases
PeroxiBase	3563. MaqCP01_VT8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4656035.
GenomeReviews	Gene locus Maqu_1056 in contig CP000514_GR.
KEGG	maq:Maqu_1056.
PATRIC	22457350. VBIMarAqu65105_1451.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
PhylomeDB	A1TZH9.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	MAQU351348:MAQU_1056-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_MARAV

Accession

Primary (citable) accession number: A1TZH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program