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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei564Aminoacyl-adenylateUniRule annotation1
Binding sitei608ATPUniRule annotation1
Metal bindingi902ZincUniRule annotation1
Metal bindingi905ZincUniRule annotation1
Metal bindingi922ZincUniRule annotation1
Metal bindingi925ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Maqu_0862
OrganismiMarinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
Taxonomic identifieri351348 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeMarinobacter
Proteomesi
  • UP000000998 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000220911 – 939Isoleucine--tRNA ligaseAdd BLAST939

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi351348.Maqu_0862.

Structurei

3D structure databases

ProteinModelPortaliA1TYY9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi605 – 609"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07. Bacteria.
COG0060. LUCA.
HOGENOMiHOG000246402.
KOiK01870.
OMAiPIPFFLH.
OrthoDBiPOG091H028I.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1TYY9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDYKHTLNL PETAFPMRGN LAKREPDMLK HWQDLNVYGN LRKQRQGREK
60 70 80 90 100
FILHDGPPYA NGSIHIGHAV NKILKDMIVK SRSFMGYDAP YVPGWDCHGL
110 120 130 140 150
PIEHKVEQEI GKAGVKVDYK TFRQACRDYA TKQIEGQKTD FIRLGVMGEW
160 170 180 190 200
DKPYLTMDPK VEAGIVRALG KIVAKGHLVR GFKPVYWSVV GQSALAEAEV
210 220 230 240 250
EYQDKTSTQI DVRFTVVDRD AVLKTFGTSE GEGDVSLVIW TTTPWTIPAN
260 270 280 290 300
QAVSIGADIE YALVQVDVGH GAERMILATD MVQGIMARWG VEDYKVLANA
310 320 330 340 350
AGSALENQLL HHPVYDKQVP VLLGDHVSLD AGTGAVHTAP DHGMEDFVVG
360 370 380 390 400
KEYGIGTLNL VKADGTYTDA AGEFAGVHVY KADEPVCAAL EREGKLVRSE
410 420 430 440 450
KFRHSYPHCW RTKTPLIYRA TPQWFISMDK LNLRADALEA IKGVRWVPAW
460 470 480 490 500
GQNRIEAMFE QSPDWCISRQ RTWGVPITLF IHKETQELHP NTQELIEKVA
510 520 530 540 550
QAVETGGIDA WYDIDQNELL GADADQYEKV LDTLDVWFDS GVTHDSVLRV
560 570 580 590 600
REELGQFPAD MYLEGSDQHR GWFQSSLKTS IAMNGVAPYK QVLTHGFTVD
610 620 630 640 650
AKGYKMSKSL GNVIAPQEVM NELGADILRL WVSATDYSGE MSVSKDILRQ
660 670 680 690 700
TADGYRRIRN TARFLLSNLT GFDPDQHMVA PEDMIALDRW MVDRALQLQN
710 720 730 740 750
ELHEDYGNYA FLRIYQKVYN FCEATLGGFY LDIIKDRQYT TQADSLARRS
760 770 780 790 800
CQTALYHVAE ALVRWIAPIL SFTADEIWQH LPGKRGDTVF YETWYEGLTA
810 820 830 840 850
LPEGFELGRD YWREIYAVKE AVNKCLEEVR ARGEIKGSLS AEVTLYCEGS
860 870 880 890 900
LAERLNYLGE ELRFVLITSE ATVKPVSEAA GVEQTNLEGL LVKVTPATHA
910 920 930
KCERCWHHRE DVGQNEQYSD LCGRCVTNVE GPGETRAYA
Length:939
Mass (Da):105,741
Last modified:February 6, 2007 - v1
Checksum:iDA9F41B2A0343794
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000514 Genomic DNA. Translation: ABM17958.1.
RefSeqiWP_011784380.1. NC_008740.1.

Genome annotation databases

EnsemblBacteriaiABM17958; ABM17958; Maqu_0862.
KEGGimaq:Maqu_0862.
PATRICi22456948. VBIMarAqu65105_1260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000514 Genomic DNA. Translation: ABM17958.1.
RefSeqiWP_011784380.1. NC_008740.1.

3D structure databases

ProteinModelPortaliA1TYY9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi351348.Maqu_0862.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABM17958; ABM17958; Maqu_0862.
KEGGimaq:Maqu_0862.
PATRICi22456948. VBIMarAqu65105_1260.

Phylogenomic databases

eggNOGiENOG4105C07. Bacteria.
COG0060. LUCA.
HOGENOMiHOG000246402.
KOiK01870.
OMAiPIPFFLH.
OrthoDBiPOG091H028I.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYI_MARHV
AccessioniPrimary (citable) accession number: A1TYY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: November 2, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.