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A1TYY9

- SYI_MARHV

UniProt

A1TYY9 - SYI_MARHV

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylateUniRule annotation
Binding sitei608 – 6081ATPUniRule annotation
Metal bindingi902 – 9021ZincUniRule annotation
Metal bindingi905 – 9051ZincUniRule annotation
Metal bindingi922 – 9221ZincUniRule annotation
Metal bindingi925 – 9251ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMHYD351348:GHYZ-876-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Maqu_0862
OrganismiMarinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
Taxonomic identifieri351348 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeMarinobacter
ProteomesiUP000000998: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 939939Isoleucine--tRNA ligasePRO_1000022091Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi351348.Maqu_0862.

Structurei

3D structure databases

ProteinModelPortaliA1TYY9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi605 – 6095"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKKRIELM.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1TYY9-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MSDYKHTLNL PETAFPMRGN LAKREPDMLK HWQDLNVYGN LRKQRQGREK
60 70 80 90 100
FILHDGPPYA NGSIHIGHAV NKILKDMIVK SRSFMGYDAP YVPGWDCHGL
110 120 130 140 150
PIEHKVEQEI GKAGVKVDYK TFRQACRDYA TKQIEGQKTD FIRLGVMGEW
160 170 180 190 200
DKPYLTMDPK VEAGIVRALG KIVAKGHLVR GFKPVYWSVV GQSALAEAEV
210 220 230 240 250
EYQDKTSTQI DVRFTVVDRD AVLKTFGTSE GEGDVSLVIW TTTPWTIPAN
260 270 280 290 300
QAVSIGADIE YALVQVDVGH GAERMILATD MVQGIMARWG VEDYKVLANA
310 320 330 340 350
AGSALENQLL HHPVYDKQVP VLLGDHVSLD AGTGAVHTAP DHGMEDFVVG
360 370 380 390 400
KEYGIGTLNL VKADGTYTDA AGEFAGVHVY KADEPVCAAL EREGKLVRSE
410 420 430 440 450
KFRHSYPHCW RTKTPLIYRA TPQWFISMDK LNLRADALEA IKGVRWVPAW
460 470 480 490 500
GQNRIEAMFE QSPDWCISRQ RTWGVPITLF IHKETQELHP NTQELIEKVA
510 520 530 540 550
QAVETGGIDA WYDIDQNELL GADADQYEKV LDTLDVWFDS GVTHDSVLRV
560 570 580 590 600
REELGQFPAD MYLEGSDQHR GWFQSSLKTS IAMNGVAPYK QVLTHGFTVD
610 620 630 640 650
AKGYKMSKSL GNVIAPQEVM NELGADILRL WVSATDYSGE MSVSKDILRQ
660 670 680 690 700
TADGYRRIRN TARFLLSNLT GFDPDQHMVA PEDMIALDRW MVDRALQLQN
710 720 730 740 750
ELHEDYGNYA FLRIYQKVYN FCEATLGGFY LDIIKDRQYT TQADSLARRS
760 770 780 790 800
CQTALYHVAE ALVRWIAPIL SFTADEIWQH LPGKRGDTVF YETWYEGLTA
810 820 830 840 850
LPEGFELGRD YWREIYAVKE AVNKCLEEVR ARGEIKGSLS AEVTLYCEGS
860 870 880 890 900
LAERLNYLGE ELRFVLITSE ATVKPVSEAA GVEQTNLEGL LVKVTPATHA
910 920 930
KCERCWHHRE DVGQNEQYSD LCGRCVTNVE GPGETRAYA
Length:939
Mass (Da):105,741
Last modified:February 6, 2007 - v1
Checksum:iDA9F41B2A0343794
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000514 Genomic DNA. Translation: ABM17958.1.
RefSeqiWP_011784380.1. NC_008740.1.
YP_958145.1. NC_008740.1.

Genome annotation databases

EnsemblBacteriaiABM17958; ABM17958; Maqu_0862.
GeneIDi4655096.
KEGGimaq:Maqu_0862.
PATRICi22456948. VBIMarAqu65105_1260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000514 Genomic DNA. Translation: ABM17958.1 .
RefSeqi WP_011784380.1. NC_008740.1.
YP_958145.1. NC_008740.1.

3D structure databases

ProteinModelPortali A1TYY9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 351348.Maqu_0862.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABM17958 ; ABM17958 ; Maqu_0862 .
GeneIDi 4655096.
KEGGi maq:Maqu_0862.
PATRICi 22456948. VBIMarAqu65105_1260.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KKRIELM.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MHYD351348:GHYZ-876-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Marinobacter aquaeolei VT8."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.
    , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Edwards K., Richardson P.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700491 / DSM 11845 / VT8.

Entry informationi

Entry nameiSYI_MARHV
AccessioniPrimary (citable) accession number: A1TYY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: November 26, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3