A1TVC1 (ACEK_ACIAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase kinase/phosphatase
Short name=IDH kinase/phosphatase
Short name=IDHK/P
EC=2.7.11.5
EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Aave_4369

Organism

Acidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the AceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: EC phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 602	602	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288277
Regions
	Nucleotide binding	325 – 331	7	ATP By similarity
Sites
	Active site	381	1	By similarity
	Binding site	346	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TVC1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 745D93F92FD561D5	FASTA	602	70,101
	10 20 30 40 50 60 MFPRRLDSPA AYGIAQAMMD GFDRHYRLFR AESARAKHRF ETSDWSGQQR AQRERIEFYD 70 80 90 100 110 120 LRVKECVRRL EKEFSAGAQP MDVWHQVKLH YIGLLVGHRQ PELAETFFNS VTTKILHRTH 130 140 150 160 170 180 FHNDFIFVRP AISTEYLEND EPGARPTYRA YYPTPESLQE TLVRVVDNFQ LQGEFEDLAR 190 200 210 220 230 240 DAGRVAEVMR PRLGPAKWRA NFQIQVLSSL FYRNKGAYLV GKVINGFQEL AFALPILHGT 250 260 270 280 290 300 DGRYVIDAAL FGEDDLQMLF SFARAYFMVD MEIPSAYVQF LRSLMPRKPR AELYNALGLA 310 320 330 340 350 360 KQGKTLFYRD FLHHLRYSSD RFRIAPGIKG MVMLVFDLPS FPFVFKVIKD YYPPQKDTTR 370 380 390 400 410 420 EQIKGKYLLV KQHDRVGRMA DTLEYSEVAF PRERFEDDLI AEIEKFAPSQ LEISDRDGDG 430 440 450 460 470 480 NVEVILKHVY IERRMIPLNI YLQEAFDAGP HDARARQQME RSVVEYGNAI KDLVAANIFP 490 500 510 520 530 540 GDMLWKNFGV TRNGKVVFYD YDEIEYLTDC HFRRVPAPRN EEDELSGEVW WAVGPRDVFP 550 560 570 580 590 600 ETFGPFLLGN DAVREVFMRH HADLLDPVFW QSHKERIQAG HMYDVFPYDP ERRFGAGTGS PA

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AAC00-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000512 Genomic DNA. Translation: ABM34909.1.
RefSeq	YP_972683.1. NC_008752.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1TVC1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4668089.
GenomeReviews	Gene locus Aave_4369 in contig CP000512_GR.
KEGG	aav:Aave_4369.
NMPDR	fig|397945.5.peg.3753.
PATRIC	20683957. VBIAciCit38535_4443.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
PhylomeDB	A1TVC1.
ProtClustDB	PRK02946.
Enzyme and pathway databases
BioCyc	AAVE397945:AAVE_4369-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
KO	K00906.
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_ACIAC

Accession

Primary (citable) accession number: A1TVC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program