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A1TTU1 (SYM_ACIAC) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase
EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:Aave_3834
OrganismAcidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli) [Complete proteome] [HAMAP]
Taxonomic identifier397945 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length706 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098

Subunit structure

Homodimer By similarity. HAMAP MF_00098

Subcellular location

Cytoplasm By similarity HAMAP MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 706706Methionine--tRNA ligase HAMAP MF_00098
PRO_0000331771

Regions

Domain600 – 706107tRNA-binding
Motif13 – 2311"HIGH" region HAMAP MF_00098
Motif341 – 3455"KMSKS" region HAMAP MF_00098

Sites

Metal binding1441Zinc By similarity
Metal binding1471Zinc By similarity
Metal binding1571Zinc By similarity
Metal binding1601Zinc By similarity
Binding site3441ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1TTU1 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 326E469A73365E41

FASTA70677,578
        10         20         30         40         50         60 
MPARKIFVTT ALPYANGNFH IGHIMEYIQA DIWVRFQRMQ GAEVNFVGAD DTHGAPIMIA 

        70         80         90        100        110        120 
AEKAGKTPQQ FVADIAAGRK PYLEGFHIRF DNWHSTDAPE NHELARQIYR DLQAAGLIET 

       130        140        150        160        170        180 
RTIEQFFDPE KNMFLPDRFI KGECPRCHAR DQYGDNCENC GAVYAPTDLI EPYSALSGAK 

       190        200        210        220        230        240 
PVLKSSDHFF FQLSDPRCVA FLQEWTQDGR LQPEVANKVK EWFSVRTNPD GTTSEGLGDW 

       250        260        270        280        290        300 
DISRDAPYFG IEIPDAPGKY FYVWLDAPVG YLASLKNLLE KRGQSYDDYV ADPQLEQVHF 

       310        320        330        340        350        360 
IGKDIVTFHT LFWPAMLKFS GRKTPDAVFV HGFLTVNNGE KMSKSRGTGL DPLKYLGLGM 

       370        380        390        400        410        420 
NAEWLRYYLA AKLNGRNEDI DFNAEDFMAR VNSDLIGKFV NIASRAAGFL TKRFGGRLGA 

       430        440        450        460        470        480 
PDADGAALLE ALRAQAGAIA EAYERRDTAR AVRETMLLAD RVNEYVDARK PWELAKQEGQ 

       490        500        510        520        530        540 
EAALQAACTT CIEAFRLLTL YLKPVLPALA AQVEAFLNVQ PLTFADAPAL LGEGHAIGAY 

       550        560        570        580        590        600 
QHLMQRVDIK QLEALFEVPA AAAAPAAPAA NAAAEASAAA GAATEAPGGE HIAPTITIDD 

       610        620        630        640        650        660 
FAKIDLRIAL IVNCEPVEGS TKLLRLTLDV GEGRHRNVFS GIASAYRPEE LVGKLTVMVA 

       670        680        690        700 
NLAPRKMKFG VSEGMVLAAS HGDEKAHPGI HVLNPWPGAT PGMRVR

« Hide

References

[1]"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AAC00-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000512 Genomic DNA. Translation: ABM34379.1.
RefSeqYP_972153.1. NC_008752.1.

3D structure databases

ProteinModelPortalA1TTU1.
SMRA1TTU1. Positions 3-557.
ModBaseSearch...

Protein-protein interaction databases

STRINGA1TTU1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4667700.
GenomeReviewsGene locus Aave_3834 in contig CP000512_GR.
KEGGaav:Aave_3834.
NMPDRfig|397945.5.peg.3270.
PATRIC20682833. VBIAciCit38535_3882.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0143.
HOGENOMHBG721667.
OMARASDHAY.
PhylomeDBA1TTU1.
ProtClustDBPRK00133.

Enzyme and pathway databases

BioCycAAVE397945:AAVE_3834-MONOMER.

Family and domain databases

HAMAPMF_00098. Met_tRNA_synth_type1.
[Tree]
InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023458. Met-tRNA_synth_1.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01874.
PfamPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. MetG. 1 hit.
TIGR00399. MetG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_ACIAC
AccessionPrimary (citable) accession number: A1TTU1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: February 6, 2007
Last modified: January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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