Reviewed, UniProtKB/Swiss-Prot A1TTM6 (SYS_ACIAC)

Names and origin

Protein names

Recommended name:
    Seryl-tRNA synthetase
    EC=6.1.1.11
Alternative name(s):
    Seryl-tRNA(Ser/Sec) synthetase
    Serine--tRNA ligase
      Short name=SerRS

Gene names

Name:	serS
Ordered Locus Names:	Aave_3767

Organism

Acidovorax avenae subsp. citrulli (strain AAC00-1) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity.
Catalytic activity	ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). HAMAP MF_00176 ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec). HAMAP MF_00176
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. HAMAP MF_00176
Subunit structure	Homodimer. The tRNA molecule binds across the dimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	selenocysteine biosynthetic process Inferred from electronic annotation. Source: HAMAP seryl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP serine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Seryl-tRNA synthetase HAMAP MF_00176		PRO_1000019599
Regions
	Nucleotide binding	278 – 280	3	ATP By similarity
	Nucleotide binding	365 – 368	4	ATP By similarity
	Region	247 – 249	3	Serine binding By similarity
Sites
	Binding site	301	1	Serine By similarity
	Binding site	400	1	Serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TTM6-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: D561939AE4BF4DFF	FASTA	439	48,488
	10 20 30 40 50 60 MLDILLLRKD LDSAIARLET RKKPQAFLQV EAFQSLESER KTLQTRTEEL QSRRNQLSKQ 70 80 90 100 110 120 VGQLKARGEN ADAVMAEVGG LKAELENSAA RLEQIQAELH TLLVAVPNLP HESVPVGSDE 130 140 150 160 170 180 SGNVEVRRWS PDGQDPKPLG FPAKDHVDLG EPLGLDFDMG VKLSGSRFTV MKGPIARLHR 190 200 210 220 230 240 ALAQFMLDVQ TREHGYTECY VPYVVNADSL RGTGQLPKFE GDLFAAKKGG QDGEPVPDNA 250 260 270 280 290 300 ALYLIPTSEV PLTNFVRDQV VPESELPIRL TAHTPCFRSE AGSYGRDTRG MIRQHQFDKV 310 320 330 340 350 360 EMVQITHPDR SYEALEEMTG HAEAVLQKLG LPYRVMSLCT GDMGFGAAKT YDLEVWLPAQ 370 380 390 400 410 420 NTYREISSVS NCEAFQARRL QARFKNAQGK NELVHTLNGS GLAVGRTLVA VLENYQQADG 430 SVAVPEALRP YLGGDSVLR

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000512 Genomic DNA. Translation: ABM34314.1.
RefSeq	YP_972088.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1TTM6.
Genome annotation databases
GeneID	4668575.
GenomeReviews	Gene locus Aave_3767 in contig CP000512_GR.
KEGG	aav:Aave_3767.
NMPDR	fig|397945.5.peg.3218.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EHFEIGE.
Family and domain databases
HAMAP	MF_00176. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002317. Ser-tRNA-synth_IIa. IPR018156. Ser-tRNA-synth_IIa_C. IPR015866. Ser-tRNA-synth_IIa_N. [Graphical view]
Gene3D	G3DSA:1.10.287.40. Ser-tRNA-synth_IIa_N. 1 hit.
PANTHER	PTHR11778. tRNA-synt_ser. 1 hit.
Pfam	PF02403. Seryl_tRNA_N. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTS	PR00981. TRNASYNTHSER.
TIGRFAMs	TIGR00414. serS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYS_ACIAC

Accession

Primary (citable) accession number: A1TTM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)