A1TT99 (SYD_ACIAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	Aave_3637

Organism

Acidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	604 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 604	604	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000006624

Sequences

	Sequence		Length	Mass (Da)
	A1TT99 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 4E8627A04A536535	FASTA	604	67,975
	10 20 30 40 50 60 MAMRSHYCGL VTEALMGQTV TLAGWVNRRR DHGGVIFIDL RDREGNVQVV CDPDRADMFK 70 80 90 100 110 120 TAEGVRNEFC VQVKGLVRAR PEGTTNDSLK SGRIEVLCHE LNVLNPSVTP PFQMDDENLS 130 140 150 160 170 180 ETTRLTHRVL DLRRPYMQRN LMLRYKTAIQ VRNFLDKEGF IDIETPMLGK STPEGARDYL 190 200 210 220 230 240 VPSRVHDGQF FALPQSPQLY KQMLMVAGYD RYYQITKCFR DEDLRADRQP EFTQIDCETS 250 260 270 280 290 300 FLNEEEIRAI FQRMVTEVFK TQLDVDLGEF PIMTYQDAAF RFGSDKPDLR VKLEFTELTE 310 320 330 340 350 360 VMKDVDFKVF SGAANMQGGR VVALRVPGGA RENGGLSRGE IDAYTEFVKI YGAKGLAYIK 370 380 390 400 410 420 VNELAKGRDG LQSPIVKNIH DAAIAEILKR TGAQDGDLLF FGADKTKVVN DAIGALRLKI 430 440 450 460 470 480 GHSEFGRKNG LFENVWAPLW VVDFPMFEYD EDDARWVAVH HPFTSPKDGH EDLMDTDPGK 490 500 510 520 530 540 CLAKAYDMVL NGWELGGGSV RIHRAEVQSK VFAALKLTPE DARAKFGYLL DALQYGAPPH 550 560 570 580 590 600 GGLAFGLDRL ITLMTGAESI RDVIAFPKTQ RAQDLLTQAP SPVDEKQLRE LHIRLRNPDA AKAA

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AAC00-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000512 Genomic DNA. Translation: ABM34187.1.
RefSeq	YP_971961.1. NC_008752.1.
3D structure databases
ProteinModelPortal	A1TT99.
SMR	A1TT99. Positions 3-596.
ModBase	Search...
Protein-protein interaction databases
STRING	A1TT99.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4665942.
GenomeReviews	Gene locus Aave_3637 in contig CP000512_GR.
KEGG	aav:Aave_3637.
NMPDR	fig|397945.5.peg.3103.
PATRIC	20682437. VBIAciCit38535_3687.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0173.
HOGENOM	HBG396032.
OMA	AFPKTQQ.
PhylomeDB	A1TT99.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	AAVE397945:AAVE_3637-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_ACIAC

Accession

Primary (citable) accession number: A1TT99

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program