A1TRV8 (KATG_ACIAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Aave_3132

Organism

Acidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 743	743	Catalase-peroxidase HAMAP MF_01961		PRO_0000354710
Sites
	Active site	94	1	Proton acceptor By similarity
	Metal binding	277	1	Iron (heme axial ligand) By similarity
	Site	90	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	93 ↔ 236		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-262) By similarity
	Cross-link	236 ↔ 262		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-93) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TRV8 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: F39D462DCA067D92	FASTA	743	81,229
	10 20 30 40 50 60 MTQDATKCPF HAAAASGGII NKDWWPNQLR LDLLNQHSSK SDPLGAGFDY AEEFKKLDYE 70 80 90 100 110 120 GLKRDLRALM TDSQDWWPAD FGHYGPQFVR MTWHAAGTYR TGDGRGGAGR GQQRFAPLNS 130 140 150 160 170 180 WPDNVNIDKS RRLLWPIKQK YGQKISWADL LVLTGNVALE TMGFRTFGFA GGREDTWEPD 190 200 210 220 230 240 QDVYWGSEKT WLGGDARYGK GTPGDGTLVA EPAMHGHEKD RVLENPLAAV QMGLIYVNPE 250 260 270 280 290 300 GPEGNPDPVA AAHDIRETFA RMAMNDEETV ALIAGGHTFG KTHGAGTADH VGPEPEAAGL 310 320 330 340 350 360 EQQGLGWASS FGSGKAGDAI TSGLEVTWTS TPAQWGNEFF ENLFKFEWEL TKSPAGAHQW 370 380 390 400 410 420 QAKNAPATIP DAHDPSKKRV PTMLTTDLSL RFDPAYEKIS RRFLENPQAF AEAFARAWFK 430 440 450 460 470 480 LTHRDMGPRA RYLGPEVPKE ELVWQDPIPA VTHDLVDAAD VAALKARVLA SGLTVQELVG 490 500 510 520 530 540 TAWASASTFR GSDKRGGANG ARIRLAPQKD WAVNQPEQLA RVLQVLEGIQ REFNKGADGD 550 560 570 580 590 600 KRISLADLIV LAGAAGVEQA ARAAGVAVTV PFHPGRADAR QDQTDVESFS VLEPSADGFR 610 620 630 640 650 660 NYTKARYSVP AEVLLLDKAQ LLTLTAPEMT VLVGGLRAIN INVGGATHGV FTATPGALTN 670 680 690 700 710 720 DFFANLLDMS TEWKPAGDLF EGRDRKTGEK RWTGTRVDLV FGSNSVLRSL AEVYASADAK 730 740 EKFVRDFVAA WAKVMDLDRF DLR

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AAC00-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000512 Genomic DNA. Translation: ABM33696.1.
RefSeq	YP_971470.1. NC_008752.1.
3D structure databases
ProteinModelPortal	A1TRV8.
SMR	A1TRV8. Positions 18-742.
ModBase	Search...
Protein-protein interaction databases
STRING	A1TRV8.
Protein family/group databases
PeroxiBase	5384. AavCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4668045.
GenomeReviews	Gene locus Aave_3132 in contig CP000512_GR.
KEGG	aav:Aave_3132.
NMPDR	fig|397945.5.peg.2670.
PATRIC	20681431. VBIAciCit38535_3189.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	KRHAPSM.
PhylomeDB	A1TRV8.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	AAVE397945:AAVE_3132-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_ACIAC

Accession

Primary (citable) accession number: A1TRV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program