A1TRL4 (SYA_ACIAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Aave_3035

Organism

Acidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	948 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 948	948	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347471
Sites
	Metal binding	638	1	Zinc Potential
	Metal binding	642	1	Zinc Potential
	Metal binding	739	1	Zinc Potential
	Metal binding	743	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A1TRL4 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 15942A7F6D276DE1	FASTA	948	102,424
	10 20 30 40 50 60 MTKPTPPSSV ADIRKSFLDF FASKGHTVVP SSPLVPGNDP TLMFTNSGMV QFKDVFLGTD 70 80 90 100 110 120 KRPYVRATSV QACLRAGGKH NDLENVGYTA RHHTFFEMLG NWSFGDYFKR DSLKWAWELL 130 140 150 160 170 180 TEVYGLPKER LLATVYAEDD EAYDIWTKEI GLPPERVIRI GDNKGGRYKS DNFWMMADTG 190 200 210 220 230 240 PCGPCSEIFY DHGPHIPGGP PGSPDEDGDR FIEIWNNVFM QFDMAEDGSV TPLPAPCVDT 250 260 270 280 290 300 GMGLERLAAI LQHVHSNYEI DLFAALIQAA ARETGTADLA NPSLKVIADH IRATAFLVSD 310 320 330 340 350 360 GVIPSNEGRG YVQRRIVRRA IRHGYKLGRK TPFFHSLVKD LVAQMGDAYP KLREQEQRIT 370 380 390 400 410 420 EVLKAEEERF FETLANGMDL LDSALDIQLA SKALQNKTLR WFASEGREEK LVSFKDDVQV 430 440 450 460 470 480 AEAVLISDEL RSTEYMQRLK ETVPGLNEVK TLHSVIRDWS GLTLPGDLAF KLHDTYGFPL 490 500 510 520 530 540 DLTNDVCRER GVTVDEDGFK AAMDRQKAQA RAAGKFKMDK ALEYGGEANR FSGYDGLTES 550 560 570 580 590 600 AKIVAIYVDG TSAQALEAGQ NGVVVLDNTP FYAESGGQVG DQGVIHAGGA RFAVDDTLKI 610 620 630 640 650 660 RADVYGHHGR LESGTLRVGD AVQAEVDAAL RAATMRNHSV THIMHKALRE VLGSHVQQKG 670 680 690 700 710 720 SLVNAERTRF DFAHNAPVTD AQIREIERRV NEEILANTPT GARVMDIESA QKTGAMMLFG 730 740 750 760 770 780 EKYGETVRVL DIGTSRELCG GTHVARTGDI GLFKVVGESG VAAGVRRIEA VTGAGALAYL 790 800 810 820 830 840 QQLEDTVAKA AGALRAPAAE ITGRIGQALE QVKALEREVA ALKGKLASSQ GDELAGQAVD 850 860 870 880 890 900 VKGLKVLAAT LPGADAKTLR DTMDKLKDKL KSAAIVLAAV DGAKVQIAAG VTPDAMAKVK 910 920 930 940 AGELVNFVAS QVGGKGGGKP DMAMAGGTDA AALPAALASV AAWVGERA

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AAC00-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000512 Genomic DNA. Translation: ABM33602.1.
RefSeq	YP_971376.1. NC_008752.1.
3D structure databases
ProteinModelPortal	A1TRL4.
ModBase	Search...
Protein-protein interaction databases
STRING	A1TRL4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4666376.
GenomeReviews	Gene locus Aave_3035 in contig CP000512_GR.
KEGG	aav:Aave_3035.
NMPDR	fig|397945.5.peg.2584.
PATRIC	20681225. VBIAciCit38535_3088.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0013.
HOGENOM	HBG354397.
OMA	MFTNSGM.
PhylomeDB	A1TRL4.
Enzyme and pathway databases
BioCyc	AAVE397945:AAVE_3035-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 2 hits. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 2 hits.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ACIAC

Accession

Primary (citable) accession number: A1TRL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program