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A1TRL2 (TRMB_ACIAC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Aave_3033
OrganismAcidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli) [Complete proteome] [HAMAP]
Taxonomic identifier397945 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax

Protein attributes

Sequence length249 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 249249tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288112

Regions

Region162 – 1676Interaction with RNA Potential
Region227 – 2304Substrate binding By similarity

Sites

Active site1561 By similarity
Binding site811S-adenosyl-L-methionine By similarity
Binding site1061S-adenosyl-L-methionine By similarity
Binding site1331S-adenosyl-L-methionine By similarity
Binding site1561S-adenosyl-L-methionine By similarity
Binding site1601Substrate By similarity
Binding site1921Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A1TRL2 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 3DC2A3556C6F50A6

FASTA24927,270
        10         20         30         40         50         60 
MHSIPADTGH TPSRAPAGNG SPPADVAFPK AIKSYVRRAG RTTTGQAKAL ETFGSQFVLD 

        70         80         90        100        110        120 
YTPGPLDAQA AFGRAAPLIL EIGFGMGEAT AHIAGVRPQD DFLCCEVHEP GVGALLKRIG 

       130        140        150        160        170        180 
ERGLTNIRIL QHDAVEVIDH MLPEGSLDGV HIFFPDPWHK KRHNKRRLVQ PPLVAKLARR 

       190        200        210        220        230        240 
LKPGGYLHCA TDWQPYAEQM LEVLSAEPLL ANSADGYAPQ PDYRPLTKFE NRGLRLGHGV 


WDILFRRVA 

« Hide

References

[1]"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AAC00-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000512 Genomic DNA. Translation: ABM33600.1.
RefSeqYP_971374.1. NC_008752.1.

3D structure databases

ProteinModelPortalA1TRL2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING397945.Aave_3033.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM33600; ABM33600; Aave_3033.
GeneID4666851.
KEGGaav:Aave_3033.
PATRIC20681221. VBIAciCit38535_3086.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAHSTLEMA.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycACIT397945:GI5W-3069-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_ACIAC
AccessionPrimary (citable) accession number: A1TRL2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 6, 2007
Last modified: June 11, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways