Reviewed, UniProtKB/Swiss-Prot A1TRE0 (HUTI_ACIAC)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	Aave_2961

Organism

Acidovorax avenae subsp. citrulli (strain AAC00-1) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Imidazolonepropionase HAMAP MF_00372		PRO_0000306420
Sites
	Metal binding	81	1	Zinc or iron By similarity
	Metal binding	83	1	Zinc or iron By similarity
	Metal binding	251	1	Zinc or iron By similarity
	Metal binding	326	1	Zinc or iron By similarity
	Binding site	90	1	Substrate By similarity
	Binding site	103	1	Substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	186	1	Substrate By similarity
	Binding site	254	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TRE0-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 872B70434FD98717	FASTA	416	43,898
	10 20 30 40 50 60 MQPTPFSSLS SADGCWTGLR LAQGLFIPDV AVPDDALSCV VVQGGTVRWV GPVAAVPPAF 70 80 90 100 110 120 AALPRHAGGG ALATPGLVDC HTHLVYGGHR ANEFAMRLAG ASYEEVARAG GGIVSSVKAT 130 140 150 160 170 180 RAASEDELFA QALPRLQALL DEGVCAIEIK SGYGLALEHE RKQLRAARRL GEACGVTVRT 190 200 210 220 230 240 TFLGAHALPP EYAGRSQDYI DLVCREMLPA LAEEGLVDAV DVFCERIAFS LAETEQVFQA 250 260 270 280 290 300 AQALGLPVKL HAEQLSDMDG ARLAARYGAL SCDHIEHLSA EGIAAMKAAG TVAVLLPGAY 310 320 330 340 350 360 YTLRDTHLPP IQALREAGVP MAVSTDHNPG TSPALSLRLM ANMACTLFRL TVPEALAGIT 370 380 390 400 410 THAARALGLQ DTHGLIAAGR PADFVLWPFA EAAELAYWFG HQPPQAIVRQ GRVVQR

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000512 Genomic DNA. Translation: ABM33528.1.
RefSeq	YP_971302.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1TRE0.
Genome annotation databases
GeneID	4668440.
GenomeReviews	Gene locus Aave_2961 in contig CP000512_GR.
KEGG	aav:Aave_2961.
NMPDR	fig|397945.5.peg.2520.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MNMACTL.
Family and domain databases
HAMAP	MF_00372. [Tree]
InterPro	IPR013108. Amidohydro_3. IPR005920. HutI. [Graphical view]
Pfam	PF07969. Amidohydro_3. 1 hit. [Graphical view]
ProDom	PD001248. Amidohydro_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_ACIAC

Accession

Primary (citable) accession number: A1TRE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)