A1TR35 (SYFA_ACIAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	Aave_2855

Organism

Acidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_1000059231
Sites
	Metal binding	271	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TR35 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 72C1C9E61876906E	FASTA	350	38,912
	10 20 30 40 50 60 MNELDSLVDA ARRMFADAAT PTDLENAKAQ FLGKAGRVTE LLKGLAQLPV EEKKSRGAAV 70 80 90 100 110 120 NVAKQAIEQA LNERRQALAD AELQAQLQAE ALDVTLPGRQ RGQGGLHPVS LTLERIEGIF 130 140 150 160 170 180 GSMGFDVADG PEIESDWFNF TALNTPEDHP ARSMHDTFYV EGGTASAPLL LRTHTSPMQI 190 200 210 220 230 240 RHAVQHVKKH RALLDAGQPM PEIRVIAPGR TYRVDSDATH SPMFHQCEGL WIGENVSFKD 250 260 270 280 290 300 LKVVFTAFCR TFFESDDLVL RFRPSFFPFT EPSAEIDIQF QDGPLAGRWL EVAGSGQVHP 310 320 330 340 350 NVVRNMGLDP EKYIGFAFGM GPDRLTMLRY GVNDLRLFFD GDIRFLSQFQ

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AAC00-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000512 Genomic DNA. Translation: ABM33423.1.
RefSeq	YP_971197.1. NC_008752.1.
3D structure databases
ProteinModelPortal	A1TR35.
ModBase	Search...
Protein-protein interaction databases
STRING	A1TR35.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4668091.
GenomeReviews	Gene locus Aave_2855 in contig CP000512_GR.
KEGG	aav:Aave_2855.
NMPDR	fig|397945.5.peg.2426.
PATRIC	20680845. VBIAciCit38535_2901.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	A1TR35.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	AAVE397945:AAVE_2855-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_ACIAC

Accession

Primary (citable) accession number: A1TR35

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program