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A1TQ53 (BIOB_ACIAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Aave_2518
OrganismAcidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli) [Complete proteome] [HAMAP]
Taxonomic identifier397945 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354Biotin synthase HAMAP-Rule MF_01694
PRO_0000381166

Sites

Metal binding791Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding831Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding861Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1231Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1541Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2141Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2861Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A1TQ53 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: A85FB4E19D0AB62F

FASTA35438,005
        10         20         30         40         50         60 
MTATLSDIRP EAPAVPVQLH RPARTPAPAA GTWSVQAVQE LLDLPFMELL WRAQSVHREH 

        70         80         90        100        110        120 
WPAGDVELAT LLSVKTGGCP ENCGYCPQSA EFDTGVKAQK LMSVDEVTTA ARAARDAGAT 

       130        140        150        160        170        180 
RFCMGAAWRA PKDRDIEKVG ELIAAVKGLG MQTCATLGML EPHQAQALKG AGLDYYNHNL 

       190        200        210        220        230        240 
DTAPEYYTDV VSTRAYQERL DTLRHVREAG ISVCCGGIIG MGEAPVHRAG LIAQLANLDP 

       250        260        270        280        290        300 
YPESVPINSL VRVPGTPLAD SDPVDPLDFV RVIAVARITM PRARVRLSAG RQQMGDAVQA 

       310        320        330        340        350 
LCFLAGANSI FYGDKLLVTG NPDVDADVQL LAKLGMNGRQ VASTEPHDLH HHAP 

« Hide

References

[1]"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AAC00-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000512 Genomic DNA. Translation: ABM33091.1.
RefSeqYP_970865.1. NC_008752.1.

3D structure databases

ProteinModelPortalA1TQ53.
SMRA1TQ53. Positions 32-340.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING397945.Aave_2518.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM33091; ABM33091; Aave_2518.
GeneID4668820.
KEGGaav:Aave_2518.
PATRIC20680179. VBIAciCit38535_2570.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycACIT397945:GI5W-2549-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_ACIAC
AccessionPrimary (citable) accession number: A1TQ53
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways