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A1TQ53

- BIOB_ACIAC

UniProt

A1TQ53 - BIOB_ACIAC

Protein

Biotin synthase

Gene

bioB

Organism
Acidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi83 – 831Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi123 – 1231Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi154 – 1541Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi214 – 2141Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi286 – 2861Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciACIT397945:GI5W-2549-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Aave_2518
    OrganismiAcidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli)
    Taxonomic identifieri397945 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax
    ProteomesiUP000002596: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 354354Biotin synthasePRO_0000381166Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi397945.Aave_2518.

    Structurei

    3D structure databases

    ProteinModelPortaliA1TQ53.
    SMRiA1TQ53. Positions 32-340.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A1TQ53-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTATLSDIRP EAPAVPVQLH RPARTPAPAA GTWSVQAVQE LLDLPFMELL    50
    WRAQSVHREH WPAGDVELAT LLSVKTGGCP ENCGYCPQSA EFDTGVKAQK 100
    LMSVDEVTTA ARAARDAGAT RFCMGAAWRA PKDRDIEKVG ELIAAVKGLG 150
    MQTCATLGML EPHQAQALKG AGLDYYNHNL DTAPEYYTDV VSTRAYQERL 200
    DTLRHVREAG ISVCCGGIIG MGEAPVHRAG LIAQLANLDP YPESVPINSL 250
    VRVPGTPLAD SDPVDPLDFV RVIAVARITM PRARVRLSAG RQQMGDAVQA 300
    LCFLAGANSI FYGDKLLVTG NPDVDADVQL LAKLGMNGRQ VASTEPHDLH 350
    HHAP 354
    Length:354
    Mass (Da):38,005
    Last modified:February 6, 2007 - v1
    Checksum:iA85FB4E19D0AB62F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000512 Genomic DNA. Translation: ABM33091.1.
    RefSeqiWP_011795618.1. NC_008752.1.
    YP_970865.1. NC_008752.1.

    Genome annotation databases

    EnsemblBacteriaiABM33091; ABM33091; Aave_2518.
    GeneIDi4668820.
    KEGGiaav:Aave_2518.
    PATRICi20680179. VBIAciCit38535_2570.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000512 Genomic DNA. Translation: ABM33091.1 .
    RefSeqi WP_011795618.1. NC_008752.1.
    YP_970865.1. NC_008752.1.

    3D structure databases

    ProteinModelPortali A1TQ53.
    SMRi A1TQ53. Positions 32-340.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 397945.Aave_2518.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABM33091 ; ABM33091 ; Aave_2518 .
    GeneIDi 4668820.
    KEGGi aav:Aave_2518.
    PATRICi 20680179. VBIAciCit38535_2570.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci ACIT397945:GI5W-2549-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1."
      Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M.
      , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.
      Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AAC00-1.

    Entry informationi

    Entry nameiBIOB_ACIAC
    AccessioniPrimary (citable) accession number: A1TQ53
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3