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A1TQ53

- BIOB_ACIAC

UniProt

A1TQ53 - BIOB_ACIAC

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Protein
Biotin synthase
Gene
bioB, Aave_2518
Organism
Acidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi83 – 831Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi123 – 1231Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi154 – 1541Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi214 – 2141Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi286 – 2861Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciACIT397945:GI5W-2549-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Aave_2518
OrganismiAcidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli)
Taxonomic identifieri397945 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax
ProteomesiUP000002596: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354Biotin synthaseUniRule annotation
PRO_0000381166Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi397945.Aave_2518.

Structurei

3D structure databases

ProteinModelPortaliA1TQ53.
SMRiA1TQ53. Positions 32-340.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A1TQ53-1 [UniParc]FASTAAdd to Basket

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MTATLSDIRP EAPAVPVQLH RPARTPAPAA GTWSVQAVQE LLDLPFMELL    50
WRAQSVHREH WPAGDVELAT LLSVKTGGCP ENCGYCPQSA EFDTGVKAQK 100
LMSVDEVTTA ARAARDAGAT RFCMGAAWRA PKDRDIEKVG ELIAAVKGLG 150
MQTCATLGML EPHQAQALKG AGLDYYNHNL DTAPEYYTDV VSTRAYQERL 200
DTLRHVREAG ISVCCGGIIG MGEAPVHRAG LIAQLANLDP YPESVPINSL 250
VRVPGTPLAD SDPVDPLDFV RVIAVARITM PRARVRLSAG RQQMGDAVQA 300
LCFLAGANSI FYGDKLLVTG NPDVDADVQL LAKLGMNGRQ VASTEPHDLH 350
HHAP 354
Length:354
Mass (Da):38,005
Last modified:February 6, 2007 - v1
Checksum:iA85FB4E19D0AB62F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000512 Genomic DNA. Translation: ABM33091.1.
RefSeqiWP_011795618.1. NC_008752.1.
YP_970865.1. NC_008752.1.

Genome annotation databases

EnsemblBacteriaiABM33091; ABM33091; Aave_2518.
GeneIDi4668820.
KEGGiaav:Aave_2518.
PATRICi20680179. VBIAciCit38535_2570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000512 Genomic DNA. Translation: ABM33091.1 .
RefSeqi WP_011795618.1. NC_008752.1.
YP_970865.1. NC_008752.1.

3D structure databases

ProteinModelPortali A1TQ53.
SMRi A1TQ53. Positions 32-340.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 397945.Aave_2518.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABM33091 ; ABM33091 ; Aave_2518 .
GeneIDi 4668820.
KEGGi aav:Aave_2518.
PATRICi 20680179. VBIAciCit38535_2570.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci ACIT397945:GI5W-2549-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M.
    , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AAC00-1.

Entry informationi

Entry nameiBIOB_ACIAC
AccessioniPrimary (citable) accession number: A1TQ53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 6, 2007
Last modified: September 3, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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