Reviewed, UniProtKB/Swiss-Prot A1TPK8 (SYC_ACIAC)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Aave_2320

Organism

Acidovorax avenae subsp. citrulli (strain AAC00-1) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00041
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Cysteinyl-tRNA synthetase HAMAP MF_00041		PRO_0000332778
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	270 – 274	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	213	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Metal binding	242	1	Zinc By similarity
	Binding site	273	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TPK8-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 326B3C9372440C95	FASTA	459	50,742
	10 20 30 40 50 60 MSLRIYNTLS RAMEDFSPLE PGHVRMYVCG MTIYDLCHIG HARMMMAFDV VQRWLRSSGL 70 80 90 100 110 120 RVTYVRNITD IDDKIIRRAV ERGITIRQLT DEMTAAMHED IGALAIEPPT VEPRATEYVP 130 140 150 160 170 180 QMLSLIGTLE RKGLAYRAGS GDVNYAVRRF EGYGKLSGKS LDELRAGERV AVQDDKQDPL 190 200 210 220 230 240 DFVLWKAAKP GEPPEAKWDS PFGTGRPGWH IECSAMSCAT LGESFDIHGG GADLQFPHHE 250 260 270 280 290 300 NEIAQSEGAT GKPLARFWMH NGFVRVDNEK MSKSLGNFFT IREVLQQVDA ETLRFFIVRT 310 320 330 340 350 360 HYRSPLNYSD VHLQDARASL KRLYTALALV APEDIGAIDW ADPHAARFKA AMDEDFGTPE 370 380 390 400 410 420 AVAVLFELAG EVNRTRSARL AGLLKALGGC LGLLQGDPQA FLRQGVQLDA AEIAARIAAR 430 440 450 AAAKAARDFA EADRIRNDLL AQGIVLKDSP AGTTWEAAQ

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000512 Genomic DNA. Translation: ABM32896.1.
RefSeq	YP_970670.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1TPK8.
Genome annotation databases
GeneID	4667407.
GenomeReviews	Gene locus Aave_2320 in contig CP000512_GR.
KEGG	aav:Aave_2320.
NMPDR	fig|397945.5.peg.2004.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VLWKAAK.
Family and domain databases
HAMAP	MF_00041. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ACIAC

Accession

Primary (citable) accession number: A1TPK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)