Reviewed, UniProtKB/Swiss-Prot A1TP96 (MDH_ACIAC)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase
    EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	Aave_2206

Organism

Acidovorax avenae subsp. citrulli (strain AAC00-1) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_01517
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 328	327	Malate dehydrogenase HAMAP MF_01517		PRO_0000294370
Regions
	Nucleotide binding	12 – 18	7	NAD By similarity
	Nucleotide binding	132 – 134	3	NAD By similarity
Sites
	Active site	190	1	Proton acceptor By similarity
	Binding site	95	1	Substrate By similarity
	Binding site	101	1	Substrate By similarity
	Binding site	108	1	NAD By similarity
	Binding site	115	1	NAD By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	165	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TP96-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: A6D2BC04ED5AD6E2	FASTA	328	34,977
	10 20 30 40 50 60 MSKKPVRVAV TGAAGQIGYA LLFRIASGEM LGKDQPVILQ LLEIPDEKAQ KALKGVMMEL 70 80 90 100 110 120 EDCAFPLLAG MEAHSDPMTA FKDTDYALLV GARPRGPGME RADLLAANAQ IFTAQGKALN 130 140 150 160 170 180 AVASRNVKVL VVGNPANTNA YIAMKSAPDL PAKNFTAMLR LDHNRAASQI AAKTGGKVGE 190 200 210 220 230 240 IEKLTVWGNH SPTMYADYRF ATIGGKSVKD AINDQVWNAD VFLPTVGKRG AAIIEARGLS 250 260 270 280 290 300 SAASAANAAI DHMRDWALGS NGKWVTMGVP SKGEYGIPEG IVFGFPVITE NGEYKIVEGL 310 320 EIDAFSQERI NKTLAELQGE QDGVKHLL

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000512 Genomic DNA. Translation: ABM32784.1.
RefSeq	YP_970558.1.
3D structure databases
SMR	A1TP96. Positions 3-328.
ModBase	Search...
Protein-protein interaction databases
STRING	A1TP96.
Genome annotation databases
GeneID	4668190.
GenomeReviews	Gene locus Aave_2206 in contig CP000512_GR.
KEGG	aav:Aave_2206.
NMPDR	fig|397945.5.peg.1904.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MIIWGNH
Family and domain databases
HAMAP	MF_01517. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR010945. Malate_DH_SF1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_ACIAC

Accession

Primary (citable) accession number: A1TP96

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	February 6, 2007
Last modified:	November 24, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)