A1TMA7 (ILVD_ACIAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Aave_1506

Organism

Acidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000000950
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	201	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A1TMA7 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: C92342C94FFDA08B	FASTA	619	66,381
	10 20 30 40 50 60 MPAYRSKTST AGRNMAGARS LWRATGMKDE DFSKPIIAVV NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AREIEAAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSRDII ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MAAMRLNIPV VFVSGGPMEA GKTRLANPVT KTIEFKKLDL VDAMVIAADD 190 200 210 220 230 240 KYSDADVAEV ERSACPTCGS CSGMFTANSM NCLTEALGLS LPGNGTVVAT HADREQLFKR 250 260 270 280 290 300 AGRRIVELAR RYYEQDDERV LPRSVGFKAF ENAMTLDIAM GGSTNTILHL LAIAQEAGID 310 320 330 340 350 360 FTMADIDRLS RVVPQLCKVA PNTNKYHIED VHRAGGIMAI LGELDRAGRL HTDAPTVHAP 370 380 390 400 410 420 TLGDALAQWD ITRTQDEAVR HFYMAGPAGI PTQVAFSQNT RWPSLDLDRA EGCIRSVEHA 430 440 450 460 470 480 FSKEGGLAVL RGNIALDGCV VKTAGVDESI HVFEGTAHVT ESQDEAVENI LADKVKAGDI 490 500 510 520 530 540 VIVRYEGPKG GPGMQEMLYP TSYIKSKGLG KACALLTDGR FSGGTSGLSI GHCSPEAAAG 550 560 570 580 590 600 GAIGLVQNGD RIRIDIPNRT IDVLVSDEEL ARRRAEQDAK GWKPAKPRPR KVSAALKAYA 610 KLVMSADKGA VRDLSLLDD

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AAC00-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000512 Genomic DNA. Translation: ABM32095.1.
RefSeq	YP_969869.1. NC_008752.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1TMA7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4667526.
GenomeReviews	Gene locus Aave_1506 in contig CP000512_GR.
KEGG	aav:Aave_1506.
NMPDR	fig|397945.5.peg.1323.
PATRIC	20678098. VBIAciCit38535_1547.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	A1TMA7.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	AAVE397945:AAVE_1506-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_ACIAC

Accession

Primary (citable) accession number: A1TMA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program