Reviewed, UniProtKB/Swiss-Prot A1TM53 (TYSY_ACIAC)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	Aave_1450

Organism

Acidovorax avenae subsp. citrulli (strain AAC00-1) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Thymidylate synthase HAMAP MF_00008		PRO_0000321463
Sites
	Active site	154	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TM53-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 7BE1EA5B552F3743	FASTA	279	31,978
	10 20 30 40 50 60 MNAPARPVRS QYEDFMRHVY THGVAKGDRT GTGTRSVFGY QMRFDLNEGF PLVTTKKVHL 70 80 90 100 110 120 KSIITELLWF LTGSSSNHWL KERGVTIWDE WAREDGDLGP VYGVQWRSWP TPDGGHIDQI 130 140 150 160 170 180 SQVVETLRTH PDSRRIIVSA WNVADLDKMA LMPCHAFFQF YVAPPQAAGE RGKLSCQLYQ 190 200 210 220 230 240 RSADIFLGVP FNIASYALLT HMMAQQCNLE VGDFIWTGGD CHIYSNHFEQ VELQLSRAPH 250 260 270 PYPTLHILRR PDSLFDYRFE DFEVRDYAHH PAIKAPVAV

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000512 Genomic DNA. Translation: ABM32041.1.
RefSeq	YP_969815.1.
3D structure databases
SMR	A1TM53. Positions 11-279.
ModBase	Search...
Protein-protein interaction databases
STRING	A1TM53.
Genome annotation databases
GeneID	4668056.
GenomeReviews	Gene locus Aave_1450 in contig CP000512_GR.
KEGG	aav:Aave_1450.
NMPDR	fig|397945.5.peg.1272.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LGPVYGH.
Family and domain databases
HAMAP	MF_00008. [Tree]
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_ACIAC

Accession

Primary (citable) accession number: A1TM53

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)