Reviewed, UniProtKB/Swiss-Prot A1TLL4 (TPIS_ACIAC)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase
      Short name=TIM
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	Aave_1261

Organism

Acidovorax avenae subsp. citrulli (strain AAC00-1) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147
Subunit structure	Homodimer By similarity. HAMAP MF_00147
Subcellular location	Cytoplasm Probable HAMAP MF_00147.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Triosephosphate isomerase HAMAP MF_00147		PRO_0000307418
Sites
	Active site	98	1	Electrophile By similarity
	Active site	169	1	Proton acceptor By similarity
	Binding site	10	1	Substrate By similarity
	Binding site	12	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TLL4-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 6F96A8F4C7DEA390	FASTA	251	26,039
	10 20 30 40 50 60 MTKKKLIAGN WKMNGTLGAN AALLQALRGG LEAGAYQNVD VAVAVPAAYL AQVQGLVEGS 70 80 90 100 110 120 AIALAAQDVS RHEAGAYTGE VSAAMLQEFG VRYVLVGHSE RRQYHGETDE QVAEKAQRAL 130 140 150 160 170 180 AAGITPVVCV GETLAEREAG QTEAVVKRQL AAVIHLNGHC ISETVVAYEP VWAIGTGRTA 190 200 210 220 230 240 TPEQAQQVHA VLRAQLAAAS EHADRIRLLY GGSMNAANAA ALLAQPDIDG GLVGGASLKA 250 QDFLQIIAAA A

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000512 Genomic DNA. Translation: ABM31852.1.
RefSeq	YP_969626.1.
3D structure databases
SMR	A1TLL4. Positions 3-251.
ModBase	Search...
Protein-protein interaction databases
STRING	A1TLL4.
Genome annotation databases
GeneID	4668329.
GenomeReviews	Gene locus Aave_1261 in contig CP000512_GR.
KEGG	aav:Aave_1261.
NMPDR	fig|397945.5.peg.1106.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0149.
HOGENOM	HBG708281.
OMA	DIRSVQT.
PhylomeDB	A1TLL4.
Family and domain databases
HAMAP	MF_00147_B. TIM_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_ACIAC

Accession

Primary (citable) accession number: A1TLL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)