Reviewed, UniProtKB/Swiss-Prot A1TKW2 (AROK_ACIAC)

Names and origin

Protein names

Recommended name:
    Shikimate kinase
      Short name=SK
    EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	Aave_1002

Organism

Acidovorax avenae subsp. citrulli (strain AAC00-1) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity.
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP MF_00109
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 183	183	Shikimate kinase HAMAP MF_00109		PRO_1000022960
Regions
	Nucleotide binding	15 – 20	6	ATP By similarity
Sites
	Metal binding	19	1	Magnesium By similarity
	Binding site	37	1	Substrate By similarity
	Binding site	61	1	Substrate By similarity
	Binding site	85	1	Substrate; via amide nitrogen By similarity
	Binding site	123	1	ATP By similarity
	Binding site	142	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1TKW2-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 25708EA14B27C233	FASTA	183	20,417
	10 20 30 40 50 60 MRGPQLSIHL IGLPGSGKST VGRHLARRLG LPFIDSDHVI EQRIGGSIRG FFDREGEAAF 70 80 90 100 110 120 RDLEEEVILE LTEANVPPQV LATGGGVVIR PGNRTRLRER GTVVYLNASP EEIFRHIRHD 130 140 150 160 170 180 QTRPLLQVGS PLDRLRELQR ERDPLYREAA HFVISPERGK NVAALVQHIA MQLELAGIRP PSP

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000512 Genomic DNA. Translation: ABM31600.1.
RefSeq	YP_969374.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1TKW2.
Genome annotation databases
GeneID	4666908.
GenomeReviews	Gene locus Aave_1002 in contig CP000512_GR.
KEGG	aav:Aave_1002.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HANVFFV.
Family and domain databases
HAMAP	MF_00109. [Tree]
InterPro	IPR000623. Shik_kinase. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_ACIAC

Accession

Primary (citable) accession number: A1TKW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)