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A1TKD3 (DDL_ACIAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Aave_0823
OrganismAcidovorax citrulli (strain AAC00-1) (Acidovorax avenae subsp. citrulli) [Complete proteome] [HAMAP]
Taxonomic identifier397945 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAcidovorax

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341044

Regions

Domain114 – 314201ATP-grasp
Nucleotide binding140 – 19556ATP By similarity

Sites

Metal binding2671Magnesium or manganese 1 By similarity
Metal binding2811Magnesium or manganese 1 By similarity
Metal binding2811Magnesium or manganese 2 By similarity
Metal binding2831Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A1TKD3 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: FD262C90DC540D1A

FASTA33435,911
        10         20         30         40         50         60 
MAAGLPETEI DVRALGKVAV LMGGDSAERE VSLMSGGGVL QALRARGVDA HAFDPSQSDL 

        70         80         90        100        110        120 
SELKSHGYAR CFIALHGRHG EDGTVQGALE LLGIPYTGPG VMASAIAMDK IMTKRIWRFE 

       130        140        150        160        170        180 
GLPTPDWRLV SSAAETAQAL EDLGSPMIVK PSREGSTIGL TKVTSPGQCE QAYRLASRYD 

       190        200        210        220        230        240 
PEVLCEQFIE GEETTCPVLG QGAGAHALPV IRIVAPEGNY DYQNKYFTDV TQYHCPCGLP 

       250        260        270        280        290        300 
EQEEREIQRL VVEAFRTLQC RGWARADIMI RASDRKPFLL EINTSPGMTG HSLVPMSARA 

       310        320        330 
MGVSYEELCL RILAGASLDA RQGQVDAAGH PGAA 

« Hide

References

[1]"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AAC00-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000512 Genomic DNA. Translation: ABM31421.1.
RefSeqYP_969195.1. NC_008752.1.

3D structure databases

ProteinModelPortalA1TKD3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING397945.Aave_0823.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABM31421; ABM31421; Aave_0823.
GeneID4668635.
KEGGaav:Aave_0823.
PATRIC20676673. VBIAciCit38535_0842.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycACIT397945:GI5W-826-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_ACIAC
AccessionPrimary (citable) accession number: A1TKD3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: February 6, 2007
Last modified: February 19, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways