Reviewed, UniProtKB/Swiss-Prot A1TKD2 (MURC_ACIAC)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Aave_0822

Organism

Acidovorax avenae subsp. citrulli (strain AAC00-1) [Complete proteome] [HAMAP]

Taxonomic identifier

397945 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Acidovorax

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000004301
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A1TKD2-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: E2586569A04F5E73	FASTA	475	50,609
	10 20 30 40 50 60 MKHAIRHIHF VGLGGAGMCG IAEVLFNLGY EISGSDLADS ATLRRLAALG IATRVGHAAA 70 80 90 100 110 120 HIEGADAVVT STAVQSDNPE VIAARERKIP VVPRALMLAE LMRLKRGIAI AGTHGKTTTT 130 140 150 160 170 180 SLVTSVLAEA GLDPTFVIGG RLNSAGANAK LGQGEYIVVE ADESDASFLN LLPVMAVVTN 190 200 210 220 230 240 IDADHMETYG HDFGRLKSAF VDFLHRMPFY GTAILCTDNP AIREILPDVT CPVTSYGFSE 250 260 270 280 290 300 DAQVRAVDVR ADAGRMRFRV QRRNGVTLPD LDVVLNLAGE HNVLNALSAI AVAVELNIPD 310 320 330 340 350 360 EALLRALAQF KGVGRRFQRY GELPAQGGGT FTLIEDYGHH PVEMTATLAA ARGAFPGRRL 370 380 390 400 410 420 VLAFQPHRYS RTRDCFEDFV KVMGSADAVL LTEVYAAGEA PIVAADGRSL ARAVRVAGQV 430 440 450 460 470 EPVFVDDIGE LPRRIADNAR GGDVVLCMGA GSIGAVPAKV VEMLRTDVPA MQEDR

References

[1]

"Complete sequence of Acidovorax avenae subsp. citrulli AAC00-1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000512 Genomic DNA. Translation: ABM31420.1.
RefSeq	YP_969194.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1TKD2.
Genome annotation databases
GeneID	4668633.
GenomeReviews	Gene locus Aave_0822 in contig CP000512_GR.
KEGG	aav:Aave_0822.
NMPDR	fig|397945.5.peg.713.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EWMVVEA.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ACIAC

Accession

Primary (citable) accession number: A1TKD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)